The response of two cotton varieties (TCH 1002 and 77/10) and two hybrids (TCHB 213 and DCH 32) to damage caused by the American bollworm, Helicoverpa armigera was evaluated under two spacings and three fertiliser levels each. Variety TCH 1002 was less damaged than 77/10, whereas between hybrids, DCH 32 was less preferred than TCHB 213. Damage was higher when the varieties were grown at a spacing of 50 × 20 cm and the hybrids at a spacing of 120 × 45 cm compared to 75 × 30 and 120 × 60 cm, respectively. Increasing the amount of fertiliser applied did not have any significant effect on damage caused by the pest. Tannin and gossypol contents were found to influence levels of damage caused by H. armigera. A combination of high tannin and gossypol resulted in reduced damage. The highest tannin content in varieties was recorded in leaves of TCH 1002 grown at a spacing of 75 × 30 cm, with a fertiliser level of 120:60:60 NPK kg/ha. The highest gossypol content was recorded in flowers of TCH 1002 under similar spacing and management levels. In the hybrids, the highest tannin and gossypol contents were recorded when DCH 32 was grown at 120 × 60 cm spacing with a fertiliser regime of 120:60:60 NPK kg/ha. Cotton was damaged to a greater extent when grown at a closer spacing. Increasing levels of nitrogen resulted in higher damage levels.