The growth regulator herbicides 2,4-D and dicamba are used to control glyphosate-resistant horseweed before crops are planted. With the impending release of 2,4-D–resistant and dicamba-resistant crops, use of these growth regulator herbicides postemergence will likely increase. The objective of this study was to determine the effectiveness of various growth regulators on Indiana horseweed populations. A greenhouse dose–response study was conducted to evaluate the effectiveness of 2,4-D ester, diglycolamine salt of dicamba, and dimethylamine salt of dicamba on control of four populations of horseweed in the greenhouse. Population 66 expressed twofold levels of tolerance to 2,4-D ester and diglycolamine salt of dicamba. Population 43 expressed an enhanced level of tolerance to diglycolamine salt of dicamba but not to the other herbicides. Diglycolamine salt of dicamba provided the best overall control of populations 3 and 34. Additionally, a field study was conducted to evaluate standard use rates of 2,4-D amine, 2,4-D ester, diglycolamine salt of dicamba, and dimethylamine salt of dicamba on control of various sized glyphosate-resistant horseweed plants. Control of plants 30 cm or less in height was 90% or greater for all four herbicides. On plants greater than 30 cm tall, diglycolamine salt of dicamba provided 97% control while 2,4-D amine provided 81% control. Diglycolamine salt of dicamba provided the highest level of control of glyphosate-resistant horseweed, followed by dimethylamine salt of dicamba, 2,4-D ester and 2,4-D amine, respectively. This research demonstrates that horseweed populations respond differently to the various salts of 2,4-D and dicamba, and it will be important to determine the appropriate use rates of each salt to control glyphosate-resistant horseweed.

In November 2004, a 29-question survey was mailed to Delaware soybean growers to determine grower perceptions of glyphosate-resistant (GR) horseweed and if glyphosate applications, GR soybean usage, and management practices had been altered in lieu of the presence of resistance. A total of 213 valid responses were received. Ninety-eight percent of respondents reported planting GR soybean at some point in the last 5 yr, with 90% reporting having planted GR soybean 3 or more years. The presence of GR horseweed on-farm was reported by 38% of the respondents and 95% of those growers with GR horseweed on-farm reported implementing one or more changes in GR soybean management. The most frequent change (66% of growers) due to resistant horseweed was the application of another herbicide with a different mode of action before planting. Forty-eight percent of growers with resistance on-farm reported a $5 to $17/ha increase to manage for GR horseweed, with 28% reporting a greater than $17/ha increase. Regardless of experience with GR horseweed, approximately 80% responded that it was worthwhile to incur additional costs now to preserve glyphosate for future use. Soybean grower reliance on glyphosate has not decreased in light of GR horseweed in Delaware. Misconceptions of timing for the selection of GR horseweed biotypes and the future availability of new herbicides with different modes of action exist within the farming community.