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footprinting modification interference

RNA (1)

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Interactions between 23S rRNA and tRNA in the ribosomal E site
M. BOCCHETTA, L. XIONG, S. SHAH, A.S. MANKIN
Journal:

RNA / Volume 7 / Issue 1 / January 2001

Published online by Cambridge University Press:

07 February 2001, pp. 54-63

Print publication:

January 2001
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Interactions between tRNA or its analogs and 23S rRNA in the large ribosomal subunit were analyzed by RNA footprinting and by modification–interference selection. In the E site, tRNA protected bases G2112, A2392, and C2394 of 23S rRNA. Truncated tRNA, lacking the anticodon stem-loop, protected A2392 and C2394, but not G2112, and tRNA derivatives with a shortened 3′ end protected only G2112, but not A2392 or C2394. Modification interference revealed C2394 as the only accessible nucleotide in 23S rRNA whose modification interferes with binding of tRNA in the large ribosomal subunit E site. The results suggest a direct contact between A76 of tRNA A76 and C2394 of 23S rRNA. Protections at G2112 may reflect interaction of this 23S rRNA region with the tRNA central fold.