Numerous threats to animal genetic resources for food and agriculture (AnGR) have been described in the literature. Yet knowledge regarding the threats facing particular breeds and production systems is patchy and often unavailable to relevant stakeholders. Lack of knowledge about threats often goes hand in hand with a more general lack of knowledge about the characteristics, use, management and distribution of livestock breeds. The study of threats should be an integral part of national surveying and monitoring strategies for AnGR. Field surveys are an opportunity to draw upon the knowledge of livestock keepers and other local stakeholders and to map breed distributions. Insights from the field should be integrated, together with information on economic trends, policy developments and the distribution of risks associated with epidemics and other disasters, into a broader understanding of threats. If a large-scale survey of stakeholder opinion is envisaged, it is important to be clear about the objectives of the exercise when designing any classification framework to be used for data collection and analysis. Analysis of threats should aim not only to record the presence or absence of particular threats but also to provide a better understanding of their spatial and temporal dynamics and how they are affected by context (location, production environment, human attitudes and objectives, etc.).