Seedlings of proso millet usually were killed by discing and rototilling in field experiments but populations were not reduced by mowing or by compressing under tractor wheels. In greenhouse experiments, recently emerged seedlings had the greatest mortality after raking and desiccation treatments but were less affected by shoot and/or root damage. In contrast, seedlings treated several weeks after emergence suffered greater mortality from shoot damage and less from desiccation. Two weedy proso millet biotypes, black and Crown, were used in the field experiments, and up to six biotypes were used in the greenhouse. Few significant differences among biotypes were found. Young (emerging) seedlings were extremely resilient to all treatments.