This article considers some of the men and women who served in the Privy Chamber of Elizabeth I and those men who held significant positions in her outer Chamber for evidence of Catholic beliefs, sympathies or family connections. It then discusses the careers of five men who at various times in Elizabeth’s reign were members of the Band of Gentlemen Pensioners. It will show that their court careers were decisively affected by their Catholic beliefs and connections and, in one case, by a temporary repudiation of Catholicism. Their careers witness both to a fluidity of religious identity that facilitated their advancement at Court and to a narrowing of this identity over the course of the reign.