Several studies have shown that feeding of an energy-dense diet over short periods to cull cows could be profitable in terms of increased saleable yield and improved carcass conditions. Although the application of growth promoters, such as anabolic implants and beta agonists, in finishing of cull cows have been recorded, there is no conclusive evidence as to the timing and duration of beta agonists in cull cow production. In this study, 288 cull cows with four or more permanent incisors and varying weights and body conditions were divided into four treatment groups so that variation in age, weight and body condition were equally distributed among groups. One group received concentrate feed without any beta agonist (C), whereas the other three groups also received concentrate feed with zilpaterol hydrochloride (6 p.p.m.) for 20 (Z20), 30 (Z30) or 40 (Z40) days, respectively, followed by a 2-day withdrawal. Animals were adapted for 10 days on a grain-based diet and fed an additional 40 days before slaughter. Growth rate and efficiency (live and carcass), trimmed meat yield and meat tenderness (Warner Bratzler shear force and sensory) of the aged (10 days) m. longissimus thoracis (LT) and m. semitendinosus (ST) were recorded. In general, Z cows had higher carcass gains and efficiency of gain than C cows (P < 0.05). In addition, Z carcasses showed higher proportional trimmed meat yields than C carcasses (P < 0.05). No significant differences in tenderness measurements were recorded for LT or ST. In general, supplementation of zilpaterol for 30 days showed better growth performance and higher trimmed meat yield than 20 and 40 days supplementation.