Archaea, once thought to be only extremophiles, are now known to be abundant in most environments. They can predominate in microbial communities and be significantly involved in many global biogeochemical cycles. However, Archaea have not been reported in Antarctic sea ice. Our understanding of the ecology of Antarctic sea ice prokaryotes is still in its infancy but this information is important if we are to understand their diversity, adaptations and biogeochemical roles in Antarctic systems. We detected Archaea in sea ice at two sampling sites taken from three subsequent years using conserved 16S rRNA gene archaeal primers and PCR. Archaeal abundance was measured using quantitative PCR and community diversity was investigated by sequencing cloned 16S rRNA gene PCR products. Archaea in Antarctic sea ice were found to be in low abundance consisting of ≤ 6.6% of the prokaryotic community. The majority, 90.8% of the sequences, clustered with the recently described phylum Thaumarchaeota, one group closely clustered with the ammonia-oxidizing Candidatus Nitrosopumilus maritimus. The remainder of the clones grouped with the Euryarchaeota.

A number of studies have suggested that high concentrations of organisms in sea ice may be the result of harvesting and concentration by frazil ice. Laboratory experiments have shown that frazil ice can concentrate organisms from two to four times above levels in the underlying water. The concentrations in nature, however, can be considerably higher. The apparent discrepancy between laboratory results and field observations can be explained by the longer temporal and spatial scales that allow more contact of ice crystals with particles and with one another in the sea. It is also likely that small-scale circulation features, such as Langmuir circulation, enhance the ability of frazil ice to concentrate organisms in a natural setting.