Studies were initiated in Wyoming to determine the potential of grass competition as an alternative to repetitive herbicide treatment or other cultural practices for control of Russian knapweed. An experiment was established to evaluate the effects of five grass species, including Russian wildrye cv. ‘Bozoisky.’ Picloram, applied to Russian knapweed during the first frost, reduced Russian knapweed from an average of 80.1% live canopy cover which equates to 0% control. Untreated, unseeded checks resulted in 83.9 and 81.1% control in tilled and nontilled treated plots, respectively. Grass cover increased in untreated seeded plots from an average of 11.3 and 8.2% in tilled and nontilled plots, respectively, to 65% in tilled and 66% in nontilled plots treated with clopyralid plus 2,4-D. Grass cover also increased 69.7% in tilled and 66% in nontilled plots treated with picloram. There was no significant difference between grass varieties when compared to percent Russian knapweed cover. Reductions to zero live canopy cover of Russian knapweed were obtained with a single application of picloram. Economic feasibility thresholds were obtained from four out of five varieties, including a significant difference provided by nontilled Russian wildrye treated with picloram.

Russian knapweed is an outcrossing perennial invasive weed in North America that can spread by both seed and horizontal rhizomic growth leading to new shoots. The predominant mode of spread at the local scale and dispersal at the long-distance scale informs control but has not been quantitatively researched. We used amplified fragment-length polymorphisms (AFLPs) of DNA collected from 174 shoots in two discrete patches of Russian knapweed at each of three locations in Montana. Out of the 174 shoots collected, we found nine AFLP genotypes. Three out of the six patches were monotypic; the other three patches each had one rare genotype. No genotypes were shared between patches. The maximum diameter of a genet (a genetic individual) was 56.5 m. These results indicate that patch expansion at the local scale is almost entirely by rhizomes that spread and develop new shoots. At the long-distance scale, dispersal is by seed. Controlling seed development through biological control and herbicide use may be effective at stopping long-distance dispersal but may not affect expansion of existing patches.