Introduction. This protocol aims at detecting and evaluating sensitivity shifts in Pseudocercospora musae (teleomorph: Mycosphaerella musicola) populations towards fungicides that are currently sprayed to control Sigatoka disease of banana. The principle, key advantages, starting plant material, time required and expected results are presented. Materials and methods. Necessary laboratory materials, and details of the seven steps of the protocol achieved during four days of experiments are described. Results. The protocol results in the observation of conidial germination of P. musae (pattern or germ tube elongation) according to the sensitivity to fungicides.