Field research was conducted in Tennessee at Jackson in 1991 and at Milan in 1992 to compare the effect of MSMA and pyrithiobac on cotton development, yield, and quality. In separate treatments to different plots, pyrithiobac at 0.14 kg ai ha–1 did not affect development, yield, and quality of cotton. MSMA at 2.24 kg ai ha–1 decreased cotton plant internode length 10 to 15% and height by 15% 5 wk after late treatment both years. MSMA applied to 50 cm cotton reduced subsequent plant height in 1992, but other plant characteristics were not affected. In 1991, MSMA increased squares and decreased blooms and bolls for monopodia and sympodia position one and two, which suggested a delay in plant development. In 1991, mechanical harvest lint yields were decreased by MSMA at first harvest while increasing second harvest lint yields. However, in 1991 only MSMA applied late decreased total harvest lint yield 20%. Plant mapping data determined that the yield decrease was a result of decreased yields at sympodia positions one and two. Cotton seed arsenic analysis indicated that MSMA-late increased arsenic levels by ≈ 1 ppmw in 1991 for sympodia at position one and two compared to the untreated check, while position two contained the highest level of 1.8 ppmw.