The macrobenthic fauna of the Rhône River below Lake Geneva was studied between 1983 and 1993 to describe patterns of longitudinal and temporal community changes. Sampling was based upon the use of artificial substrates in 11 stations. The confluence with the Arve (an alpine tributary) is the first source of faunal discontinuity, both in terms of species and feeding group composition. The Verbois hydropower plant is the second longitudinal disruption. From a temporal point of view, the construction of the Seujet hydropower plant was a major source of faunal fluctuation. After this event, and until the end of the study, lenitophilous taxa increased to the detriment of rheophilous taxa.