The newly designed HVE gas interface enables the AMS measurement of carbon samples in CO2 form. The CO2, e.g. resulting from the sample combustion in an elemental analyzer, is adsorbed in a zeolite trap and subsequently transferred to a motor-driven syringe. Once diluted with He, the gas mixture is transferred into the ion source of the AMS system. A carbon ion beam is formed in the ion source and mass-analyzed by the AMS system, resulting in 13C/12C and 14C/12C isotopic ratios. The HVE gas interface features two traps and two syringes to maximize the sample throughput, which results in more than 10 samples per hour. The first performance results of CO2 gas sample AMS measurements that were performed with the HVE gas interface in combination with the HVE 210 kV AMS system are presented in this paper. The measurements show that the gas interface contribution to the 14C/12C background is in the 10–15 level and to the precision is at or below 1%.