The light-adaptation properties of goldfish photoreceptor mechanisms were examined using Stiles' two-color threshold technique. Threshold vs. background intensity (TVI) curves were determined for isolated cone and rod mechanisms using the heart-rate conditioning technique. The principal aim of this study was to compare the light-adaptation properties of the ultraviolet (UV)-sensitive cone mechanism to the other receptor mechanisms of goldfish. This examination revealed several striking functional differences: (1) The UV-sensitive cone mechanism threshold vs. background intensity (TVI) exhibited a slope of 0.65 (compared to the approximate 1.0 for the other cone mechanisms on a log/log plot) and thus was not in accordance with Weber's law. This may in part be related to the intrusion of the blue-sensitive mechanism at the upper radiance range. (2) The operation of the UV-sensitive cone mechanism was limited to intermediate intensities (i.e. not very dim or bright). (3) The UV-sensitive cone mechanism exhibited a Weber fraction or luminance contrast threshold of 0.316 that was approximately six times larger than the other cone mechanisms but comparable to the rod mechanism. This indicates that the UV-sensitive cone mechanism performs relatively poorly in terms of brightness contrast detection.

The present study examines the changes in ultraviolet (UV) photosensitivity that occur during the growth of rainbow trout (Salmo gairdneri). A comparison of the ocular media transmission of small (n = 3) and large (n = 3) trout eyes did not reveal large changes in the transmission of UV radiation through the eye. We used the heart-rate conditioning technique to measure spectral sensitivity in immobilized trout. Four trout, each weighing less than 30 g, exhibited a UV-sensitivity peak at 360 nm while four additional trout weighing more than 60 g each exhibited no evidence of UV sensitivity. Spectral-sensitivity measurements of two trout weighing 44 g and 60 g revealed UV sensitivity, but when measured one month later (after a 25% increase in body weight) both fish exhibited no UV-sensitivity peak. At this time their sensitivity appeared to conform to the known blue-sensitive cone mechanism.

Spatial vision was studied in the bluegill sunfish, Lepomis macrochirus (9.5–14 cm standard length) to assess the limitations imposed by the optics of the eye, the retinal receptor spacing and the retinotectal projection during regeneration. Examination of images formed by the dioptric elements of the eye showed that spatial frequencies up to 29 c/° could be imaged on the retina. Cone spacing was measured in the retina of fresh, intact eyes. The spacing of rows of double cones predicted 3.4 c/° as the cutoff spatial frequency; the spacing between rows of single and double cones predicted 6.7 c/°. Contrast sensitivity functions were obtained psychophysically in normals and fish with one regenerating optic nerve. Fish were trained to orient to gratings (mean luminance = 25 cd/m2) presented to either eye. In normals, contrast sensitivity functions were similar in shape and bandwidth to those of other species, peaking at 0.4 c/° with a minimum contrast threshold of 0.03 and a cutoff at about 5 c/°, which was within the range predicted by cone spacing. Given that the optical cutoff frequency exceeds that predicted by cone spacing, it is possible that gratings could be detected by aliasing with the bluegill's regular cone mosaic. However, tests with high contrast gratings up to 15 c/° found no evidence of such detection. After crushing one optic nerve in three trained sunfish, recovery of visual avoidance, dorsal light reflex and orienting to gratings, were monitored over 315 days. At 64–69 days postcrush, responses to gratings reappeared, and within 2–5 days contrast sensitivity at low (0.15 c/°) and medium (1.0 c/°) spatial frequencies had returned to normal. At a high spatial frequency (2.93 c/°) recovery was much slower, and complete only in one fish.

Simple (dorsal light reflex) and complex (predator-prey interactions) visually mediated behaviors were used concurrently with morphological examination to assess restoration of visual function following optic nerve crush in bluegill (Lepomis macrochirus) × pumpkinseed (Lepomis gibbosus) hybrid sunfish. Regenerating optic nerve axons projected into the stratum opticum-stratum fibrosum et griseum superficiale by week 2, the stratum griseum centrale by week 4, and stratum album centrale by week 6. Initial projections into the laminae were diffuse and less stratified compared to controls. By week 12, the projection pattern of regenerating nerve fibers closely resembled the innervation of normal tecta. Visual improvements were correlated with increasing projections into the tectum. The dorsal light reflex improved from a 45° vertical deviation following nerve crush to 4.5° by week 16. Initial predator-prey interactions were exclusively mediated by the control eye. As regeneration progressed, there was a gradual expansion of the visual field. The reaction distance and attack angles within the visual field of the experimental eye were initially less than controls, however, these differences disappeared by week 10. Improvements in visual function were closely correlated with an increase of regenerating ganglion cell axons into the optic tectum indicating sufficient synaptogenesis to mediate both simple and complex visual behavior.

Neural activity in the optic tectum was compared with activity in the nucleus isthmi (NI) of both goldfish and sunfish with the aim of understanding how the two brain structures interact to process visual information. The two species yielded very similar results. Superficial tectum responds reliably to visual stimulation with topographically organized receptive fields; deep tectum and NI respond to stimulation throughout the field of the contralateral eye and habituate rapidly. Bursts of large-amplitude spiking in NI occur spontaneously and in response to contralateral visual stimulation. These NI bursts correlate with activity bursts across the tectal lobe on the same side, especially in the deeper layers. NI bursts may also synchronize with spiking activity in deep tectum. Trains of small-amplitude spikes in NI can be elicited by both ipsilateral and contralateral stimulation, but are not reflected in tectal activity. Simultaneous recordings from two sites in one NI were almost identical, suggesting that NI operates as a functional unit, broadcasting the same message across the ipsilateral tectal lobe.