We investigate in this paper the dependence relation between the space–time periodic coefficients A, q and μ of the reaction–diffusion equation
and the spreading speed of the solutions of the Cauchy problem associated with compactly supported initial data. We prove in particular that (1) taking the spatial or temporal average of μ decreases the minimal speed, (2) if μ is not constant with respect to x, then increasing the amplitude of the diffusion matrix A does not necessarily increase the minimal speed and (3) if A = IN, μ is a constant, then the introduction of a space periodic drift term q = ∇Q decreases the minimal speed. In order to prove these results, we use a variational characterisation of the spreading speed that involves a family of periodic principal eigenvalues associated with the linearisation of the equation near zero. We are thus back to the investigation of the dependence relation between this family of eigenvalues and the coefficients.