The ascidian Ecteinascidia turbinata is reported from Maltese waters for the first time. Mature colonies were recorded on artificial substrata at two different sites (and on natural substrata at one of these), 4 km apart, during the summer months. The appearance of this ascidian is expected to be seasonal as the winter temperature in Malta may fall below that required for the maintenance of zooid growth. A second species, E. moorei, which was described in 1890 is here confirmed to be the same as E. turbinata, meaning that the species has existed in the Mediterranean since at least ~1880. The possibility that the Mediterranean population is a relic one from warmer periods cannot yet be excluded, so it is best described as being cryptogenic. The species appears to be spreading slowly, perhaps as a result of the rise in surface sea temperature. The Maltese sites offer an opportunity to monitor the species as they are accessible dive sites. This will allow assessment of whether this species remains restricted to these sites, or if it spreads perhaps to impact other species.