The ascidian fauna at Campbell Bay, Bquary Beach and Laxman Beach, Andaman and Nicobar Islands, was surveyed. A total of six species – Botrylloides leachi, Botrylloides pizoni, Clavelina picta, Clavelina robusta, Phallusia julinea and Polycarpa pigmentate – were newly recorded in the Andaman and Nicobar Islands, and the genera Clavelina was recorded for the first time in India. It is likely that the Andaman and Nicobar Islands provide a better environment for the growth of ascidians and this area in particular is populated by a greater variety of these ascidians than any of the other areas of the islands. This paper details their morphological and taxonomical features.