The effects of abomasal infusion of casein or soya-bean-protein isolate (SPI) on milk production were investigated in four Friesian cows in mid-lactation receiving a basal diet of grass silage and barley which supplied energy and protein considerably in excess of requirements for milk production by conventional rationing standards. Three levels of infusion were used for each protein source, the corresponding doses being isonitrogenous for each of the proteins: 100, 220 and 330 g/d for casein and 115, 230 and 345 g/d for SPI. Casein produced much greater effects on the yield of milk and milk constituents than did SPI. On the highest dose of casein, milk yield was increased by 3.5 kg/d, fat output by 15% and protein output by 36%; corresponding values for the highest dose of SPI were 1.6 kg/d, 12% and 13% respectively. Increases in the yield of milk-protein were linear for casein but for SPI there was no increase beyond the first level of infusion. It was calculated that casein infusion had a marked effect on the utilization of energy: the increases in milk production could be explained either by a channelling of an extra 12 MJ metabolizable energy (ME)/d away from body tissue synthesis and into milk synthesis or by an increase in the efficiency of utilization of ME for lactation from 0.50 on the basal diet to 0.58; the measurements made did not allow the two mechanisms to be clearly differentiated.