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Morphological and genetic analysis of a rediscovered Clinostomum sp. parasitising Titanolebias monstrosus and Trigonectes aplocheiloides (Cyprinodontiformes: Rivulidae)

Published online by Cambridge University Press:  20 November 2024

M.M. Montes Open the ORCID record for M.M. Montes [Opens in a new window] ,
F. Alonso Open the ORCID record for F. Alonso [Opens in a new window] ,
G.E. Terán Open the ORCID record for G.E. Terán [Opens in a new window] ,
S.W. Serra Alanis Open the ORCID record for S.W. Serra Alanis [Opens in a new window] ,
M. Waldbillig Open the ORCID record for M. Waldbillig [Opens in a new window] ,
M.G. Cavallo Open the ORCID record for M.G. Cavallo [Opens in a new window] ,
D. Balcazar Open the ORCID record for D. Balcazar [Opens in a new window]  and
G.F. Reig Cardarella Open the ORCID record for G.F. Reig Cardarella [Opens in a new window]
M.M. Montes*
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina Killifish Foundation, La Plata, Buenos Aires Province, Argentina
F. Alonso
Affiliation:
Killifish Foundation, La Plata, Buenos Aires Province, Argentina Instituto de Bio y Geociencias del NOA (IBIGEO), National Scientific and Technical Research Council (CONICET). Facultad de Ciencias Naturales, Universidad Nacional de Salta (UNSa), Salta Province, Argentina
G.E. Terán
Affiliation:
Killifish Foundation, La Plata, Buenos Aires Province, Argentina Unidad Ejecutora Lillo (UEL)-CONICET-Fundación Miguel Lillo. San Miguel de Tucumán, Tucumán, Argentina
S.W. Serra Alanis
Affiliation:
Killifish Foundation, La Plata, Buenos Aires Province, Argentina Museo Nacional de Historia Natural, Montevideo, Uruguay Centro Universitario Regional del Este (CURE) Sede Rocha, Rocha, Uruguay
M. Waldbillig
Affiliation:
Killifish Foundation, La Plata, Buenos Aires Province, Argentina Instituto de Investigaciones en Producción Animal (INPA). National Scientific and Technical Research Council (CONICET). Facultad de Ciencias Veterinarias, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
M.G. Cavallo
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
D. Balcazar
Affiliation:
Centro de Estudios Parasitológicos y Vectores (CEPAVE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de La Plata (CCT, CONICET-UNLP), La Plata, Buenos Aires, Argentina
G.F. Reig Cardarella
Affiliation:
Killifish Foundation, La Plata, Buenos Aires Province, Argentina Escuela de Tecnología Médica y Centro Integrativo de Biología y Química Aplicada (CIBQA). Universidad Bernardo O’ Higgins, Santiago de Chile, Chile
*
Corresponding author: M.M. Montes; Email: [email protected]
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Abstract

Clinostomids are a group of digeneans in which substantial diversity has been recently discovered, with some metacercariae specific to their fish hosts. Genetic analysis has been instrumental in elucidating species diversity within this genus. Recently, seven COI lineages were reported in Argentina, along with three metacercarial morphotypes lacking molecular data. Two of these were found parasitising Rivulidae fishes. The discovery of Clinostomum metacercariae in Trigonectes aplocheiloides and Titanolebias monstrosus from temporary ponds in the western Chacoan region allowed us to redescribe the metacercariae previously identified by other authors and provide the first sequences of this lineage. The morphology of the metacercariae in both hosts matched previously reported descriptions. Genetic analysis clustered the new lineage with Clinostomum detruncatum, Clinostomum sp. 7, Clinostomum L1, and Clinostomum CRA. This discovery leaves only two morphological records of metacercariae to be characterised using DNA sequencing: one in another Rivulidae (Neofundulus paraguayensis) and one in a Loricaridae (Hypostomus sp.). The present results represent the eighth clinostomid lineage sequenced from Argentina, highlighting the extensive diversity in South America and the many lineages yet to be described or identified, considering that only one of these lineages is formally described based on adult specimens found in the heron Ardea cocoi.

Keywords

new lineageintegrative taxonomyCOIArgentinakillifish
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e74
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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