In human and animal nutrition, much interest has been focused on the potential role of dietary supplements in promoting health, athletic performance and disease mitigation. Supplements may include essential nutrients provided in amounts greater than required to prevent a deficiency state, or substances purported to have a role in metabolism or tissue function but that are not recognized as an essential nutrient. This review aims to provide the rationale and scientific evidence for use (or not) of some of the supplements marketed for use in horses, with emphasis on supplements purported to directly boost performance, such as creatine, carnitine and branched-chain amino acids. It also discusses the so-called ‘joint supplements’ (or slow-acting, disease-modifying osteoarthritis agents), such as glucosamine and chondroitin sulphate. The effects of selected feeding strategies on performance, including fat supplementation, are also examined. It is concluded that although the use of nutritional supplements is commonly alleged to boost performance or health in horses, for most, if not all, of these supplements there is little or no scientific evidence of efficacy.

This study tested the hypothesis that orange peel (O) and decaffeinated black tea (T) extracts would alter markers of exercise performance as well as exercise-induced mRNA expression for the inflammatory cytokines IL-6, TNF-alpha and IFN-gamma. Nine healthy, unfit Standardbred mares (age: 10±4years, ∼450kg) were assigned to three treatment groups in a randomized crossover design where each horse was administered one of the following; placebo (O; 21 water), black tea extract in water (T; 21) or orange peel extract in water (W; 21), via a nasogastric tube. One hour later the horses completed an incremental graded exercise test (GXT) on a treadmill at a fixed 6% grade with measurements and blood samples obtained at rest, at the end of each 1min step of the GXT and at 2 and 5min post-GXT. An additional set of blood samples for Polymerase Chain Reaction (PCR) measurements of mRNA was obtained before exercise and at 5 and 30min and 1, 2, 4 and 24h post-GXT. The GXTs were conducted between 0700 and 1200h not less than 7days apart. There were no differences (P>0.05) in VO2max, respiratory exchange ratio, run time, velocity at VO2max, core body temperature, haematocrit, creatine kinase (CK), plasma lactate concentrations, HR, right ventricular pressure (RVP) or pulmonary artery pressure (PAP) across treatments. A major finding was that orange peel extract significantly reduced post-exercise VO2 recovery time (W = 112±7, O = 86±6, and T = 120±11s). There was a significant difference in plasma total protein concentration (TP) in the O runs compared with water and T. TNF-alpha mRNA expression was lower in the T runs compared with water and O trials. IFN-gamma mRNA expression levels appeared to be lower in both the T and O extract runs compared with the water trials. The mRNA expression of IL-6 was unaltered across treatment groups. These data suggest that orange peel and black tea extracts may modulate the cytokine responses to intense exercise. Orange peel extract reduced post-exercise recovery time and may potentially enhance the ability of horses to perform subsequent bouts of high-intensity exercise.

Several studies have shown that the placement of a face mask on a horse can have effects on ventilation, gas exchange and the cardiovascular system during exercise. The aim of the present study was to determine if airflow and ventilation measured with the same ultrasonic flowmeters were different during exercise between horses wearing half- (HM) and full-face (FM) masks. Five clinically healthy Thoroughbred horses with no history of respiratory disease were studied in an unbalanced crossover design. They were exercised on a treadmill at speeds between 1.7 and 11ms−1 on a 3° incline wearing both masks. The following variables were recorded: peak inspired (PIF) and peak expired flow rates (PEF), inspiratory tidal volume (VT), respiratory rate (fR ), inspiratory minute ventilation (VE), inspiratory time, (TI), expiratory time (TE ), total breath time (TT), end tidal oxygen (ETO2), end tidal carbon dioxide (ETCO2) and heart rate (HR). A mask by speed of exercise interaction term was not significant for any of the models. The PEF (mean difference 12.91s−1; lower and upper 95% CI 7.6 and 18.21s−1, respectively; P<0.0001) and ETO2 (mean difference 0.77%; lower and upper 95% CI 0.48 and 1.00%, respectively; P<0.0001) were significantly greater and ETCO2 was significantly lower (mean difference −1.3%; lower and upper 95% CI −2.0 and 0.7%, respectively; P<0.0001) with the FM compared with the HM. There was also a trend for inspired VE to be higher with the FM compared with the HM (mean difference 1021min−1; lower and upper 95% CI 26 and 1781 min−1, respectively; non-significant). We conclude that the HM may impair ventilation in the horse during exercise compared with the FM, despite the latter having a greater deadspace.

Our objective was to determine if feeding a high-fat supplement versus a control supplement to growing yearling mares would affect growth an/r body composition parameters as assessed via body weight (BW), body condition scores (BCS) and concentrations of plasma hormones. Eight yearling mares were paired by initial BW (348±19kg) and maintained on pasture and supplemented with either a high-fat supplement (16% fat) or a control supplement (3% fat) at 0.8% of their BW in two daily meals for 8 weeks. Both BW and BCS increased for all mares throughout the study (each P<0.0001); however, no difference in BW or BCS could be attributed to treatment effects. Nonetheless, plasma concentrations of leptin were greater in mares fed the high-fat supplement (P = 0.0001) compared with the control supplement. Plasma concentrations of growth hormone tended to be greater in high-fat-fed mares (P = 0.06). Plasma concentrations of insulin did not differ between treatment groups (P = 0.96). Although no gross difference in BW or BCS was discernable among mares fed the control versus high-fat supplement, these data provide evidence that increasing fat content in the diet may alter leptin levels independent of changes in body composition.

Impact is considered the most critical part of the stance phase for the development of chronic articular disorders such as osteoarthritis in the equine distal limb. Modern, synthetic shoeing materials are believed to modify impact and therefore are often used to treat an/r prevent lameness due to chronic joint disorders. Scientific evidence is scarce, however, to prove this. Hoof impact of forelimb was compared quantitatively in a group of horses under three conditions: unshod, classical steel shoes and shod with a synthetic shoe. Twelve sound warmblood horses were trotted by hand on an asphalt track at a mean speed of 3.5ms−1 and measured in a Latin square design (unshod condition, with steel shoes and with polyurethane (PU) shoes) using a triaxial accelerometer that had been fixed to the lateral hoof wall of the left forelimb. The sampling frequency was set at 10kHz per channel. The maximum amplitude of vertical and horizontal, forwar/ackward accelerations at hoof impact was lowest when shod using the PU shoeing condition (P<0.05), but the duration of the impact vibrations was lowest when unshod. PU shoes cause more damping, less friction and slower shock absorption at hoof level compared with the other two conditions and thus modify impact. Synthetic, polyurethane shoes may help in reducing peak vibrations. These short-term effects appear to be promising enough to evaluate PU shoes under field conditions in reducing impact on the longer term after substantial wear and tear. Furthermore, the possible role of synthetic materials in repairing critical tissues or even in preventing osteoarthritis in horses warrants further investigation.

Pain, atrophy and dysfunction of the longissimus dorsi in the equine back can lead to poor performance and altered biomechanics. Back problems are often treated by manipulative therapy to this muscle. The purpose of this study was to identify if manipulative therapy resulted in changes to muscle tone or electromyographic (EMG) activity immediately after treatment. We measured the muscle tone during standing using a mechanical tissue indenter and the EMG activity (both at the T16 level in the longissimus dorsi) during walking around a figure-of-eight course in 26 horses. The horses were randomly assigned into three groups that received: (a) spinal (McTimoney) manipulations, (b) reflex inhibition therapy or (c) a control group that was not manipulated. The muscle tone and activity were measured immediately after treatment. Both the McTimoney and the reflex inhibition groups showed significant decreases in muscle tone (c. 12%) and walking EMG activity (c. 19%). The control group showed no significant change in tone or EMG activity. These results document how the longissimus dorsi muscle responds immediately after manipulative therapy. Further studies would be needed to identify how long such changes persist or if such changes caused a reduction in pain or an increase in performance.

Impact-related peak decelerations with high loading rates are described as indicators of mechanical stress to the limb. The hoof deceleration pattern in the beginning of the stance phase has recently been described, together with ground reaction force measurements at slow speed; however, no information is available at high speeds. The objective of the present study was to investigate the hoof deceleration pattern following impact at slow speeds, comparable to earlier force plate studies, and at high speeds on a racetrack. Eight Standardbreds equipped with triaxial accelerometers mounted on fore- and hind hooves were driven from a jogcart on a harness-training racetrack with a stone dust surface at the mean speeds of 4.7, 5.7, 10.1 and 12.7ms−1. The accelerometer signals were sampled at 10kHz during 10s of constant speed along one straight of the racetrack. The signal was studied for each speed by the analysis of representative peak decelerations. At slow trot, the deceleration pattern was similar to what was found in earlier studies over the force plate. The peak values of both the vertical and horizontal decelerations increased significantly with a higher speed (P≤0.01). It was shown that a higher speed results in changes of the hoof deceleration pattern with higher peak decelerations. The maximum horizontal deceleration, together with the second vertical peak deceleration, were suggested to be major indicators on the mechanical stress subjected to the limb.