Greenstone is commonly used to produce culturally significant items across Mesoamerica, including axes, earspools, figurines, and beads. This research characterizes the mineralogy of greenstone materials recovered from sites in the Jovel Valley, Chiapas, Mexico, to document the range of green minerals utilized by the inhabitants. Our analysis of the objects suggests that the Late Classic and Early Postclassic Maya of the Jovel Valley had access to a variety of greenstone minerals, including serpentinites, green micas, grossular, and jadeite. X-ray diffraction and X-ray fluorescence spectrometry characterization of reference materials suggests procurement of greenstone resources from the well-documented sources of the Motagua–Polochic Fault Zone, and also potentially from sources in the Chalchihuitán–Chenalhó area of Chiapas, Mexico. The Jovel Valley had access to materials over long distances through historically documented trade routes that allowed the movement of greenstone materials west from the Motagua River Valley into highland Chiapas or south from the Chalchihuitán–Chenalhó area.