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Protective efficacy of Nerium oleander extract on spermatogenesis in streptozotocin-induced diabetic rats

Published online by Cambridge University Press:  29 January 2024

Afrooz Karimi ,
Farhad Kohpeyma ,
Ebrahim Asadi ,
Maryam ziyaee  and
Samaneh Karimi Open the ORCID record for Samaneh Karimi [Opens in a new window]
Afrooz Karimi
Affiliation:
Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
Farhad Kohpeyma
Affiliation:
Endocrine and Metabolism Research Center, Shiraz University of Medical Science, Shiraz, Iran
Ebrahim Asadi
Affiliation:
Department of Biology, University of Saskatchewan, Saskatoon, SK S7N 5E2, Canada
Maryam ziyaee
Affiliation:
Abadan University of Medical Science, Abadan, Iran
Samaneh Karimi*
Affiliation:
Department of Anatomical Sciences, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran
*
Corresponding author: Samaneh Karimi; Emails: Samaneh_k_500@yahoo.com;s.karimi@abadanums.ac.ir
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Summary

Men with diabetes frequently experience spermatogenic dysfunction, which is the most significant sign that diabetes has harmed their ability to reproduce. The effect of various doses of the hydro-alcoholic extract of Nerium oleander leaves on the pituitary–gonadal axis, sperm motility and number, antioxidant system, changes in testicular tissue structure, and spermatogenesis in healthy and diabetic rats has been examined in the current study. Eighty male rats that had been streptozotocin-induced diabetic and healthy were divided into eight groups: (1) control, (2) Nerium (50 mg/kg), (3) Nerium (100 mg/kg), (4) Nerium (200 mg/kg), (5) DM (6) DM+Nerium (50 mg/kg), (7) DM+Nerium (100 mg/kg) and (8) DM+Nerium (200 mg/kg) and were administered orally for 48 days consecutive. Following the studies, analysis of the testicular tissues’ antioxidant capacity as well as sperm parameters, Johnsen’s scoring and morphometric evaluation, histology, biochemical and stereology studies were performed.

The outcomes showed that Nerium 50 and 100 mg/kg considerably enhanced the testicular morphology, sperm parameters, and reproductive organs to varying degrees in diabetic rats. After Nerium 50 mg/kg administration, glutathione peroxidase (GPX) and catalase (CAT) levels in the testicular tissue were increased whereas malondialdehyde (MDA) levels were markedly decreased. Nerium may help protect against diabetic-induced spermatogenic dysfunction in male rats by enhancing the activities of antioxidant enzymes in lower dosages.

Keywords

AntioxidantDiabetesMale ratNerium oleanderSpermatogenesis
Type
Research Article
Information
Zygote , Volume 32 , Issue 2 , April 2024 , pp. 139 - 148
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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