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Occurrence of antimicrobial resistance and class 1 integrons in Staphylococcus aureus isolated from bovine mastitis in China

Published online by Cambridge University Press:  15 October 2024

Limei Zhang ,
Kai Liu ,
Jianming Deng ,
Hui Xu ,
Juyu Wang ,
Kuan Wang ,
Weijie Qu ,
Gang Liu  and
Xiaolong Gu
Limei Zhang
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Kai Liu
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Jianming Deng
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Hui Xu
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Juyu Wang
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Kuan Wang
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Weijie Qu
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
Gang Liu
Affiliation:
College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China
Xiaolong Gu*
Affiliation:
College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province 650201, PR China
*
Corresponding author: Xiaolong Gu; Email: xuewu24588@126.com
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Abstract

Integrons are important genetic elements that allow easy acquisition and dissemination of antimicrobial resistance genes. Studies reporting occurrence of integrons in Staphylococcus aureus (S. aureus) isolated from bovine mastitis in large dairy farms across China are scarce. The aim of this study was to investigate the occurrence of class 1 integrons (intI1), antimicrobial resistance (AMR) and associated genes in S. aureus isolated from bovine mastitis and their associations. Minimum inhibitory concentrations (MICs) were determined to evaluate the AMR phenotypes, whereas PCR was carried out to assess the occurrence of AMR genes and intI1. In addition, index cluster analysis was used to estimate associations between AMR phenotype, genotype and intI1 in 103 isolates. Overall, 83% of S. aureus were intI1-positive and 5 types of gene cassettes were detected. Susceptibility against single antimicrobial agents ranged from 0% (erythromycin), 12% (ampicillin) and 16% (penicillin G) to 96% (gentamicin). Most isolates (64%) were intermediate-resistant against erythromycin, whereas resistance against ceftriaxone (22%), clindamycin (4%), cefotaxime (2%), tetracycline (1%) and ciprofloxacin (1%) were relatively uncommon. The predominant resistant gene was blaZ gene (n = 88, 85%) followed by tetD gene (n = 85, 83%). With an estimated prevalence of 12% of the mecA gene, methicillin-resistant S. aureus isolates had higher MIC50 and MIC90 for majority of antimicrobials than methicillin-susceptible S. aureus isolates. Presence of the ermC gene was associated with erythromycin resistance. Ampicillin, erythromycin and penicillin G resistance were associated with intI1. The data presented in our study indicated that class 1 integron-mediated resistance possibly plays an important role in dissemination of AMR in S. aureus isolated from bovine mastitis.

Keywords

antimicrobial resistancebovine mastitisclass 1 integronsStaphylococcus aureus
Type
Research Article
Information
Journal of Dairy Research , Volume 91 , Issue 3 , August 2024 , pp. 298 - 304
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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Footnotes

*

These authors contributed equally to this work and should be considered co-first authors.

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