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Casein composition and differential translational efficiency of casein transcripts in donkey's milk

Published online by Cambridge University Press:  30 April 2019

Gianfranco Cosenza ,
Rosalba Mauriello ,
Giuseppina Garro ,
Barbara Auzino ,
Marco Iannaccone ,
Angela Costanzo ,
Lina Chianese  and
Alfredo Pauciullo
Gianfranco Cosenza*
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Rosalba Mauriello
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Giuseppina Garro
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Barbara Auzino
Affiliation:
Department of Veterinary Sciences, University of Pisa, Pisa, Italy
Marco Iannaccone
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Angela Costanzo
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Lina Chianese
Affiliation:
Department of Agricultural Sciences, University of Naples Federico II, Portici (Naples), Italy
Alfredo Pauciullo
Affiliation:
Department of Agricultural, Forest and Food Science, University of Torino, Grugliasco (TO), Italy
*
Author for correspondence: Gianfranco Cosenza, Email: [email protected]
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Abstract

The amount of the four caseins (αs1, αs2, β and κ-CN) in donkey milk was evaluated by Urea-PAGE analysis at pH 8.6, followed by immuno-detection with polyclonal antibodies, coupled to densitometric analysis. The results showed the percentage of each casein in decreasing order: β (54.28) > αs1 (35.59) > αs2 (7.19) > κ-CN (2.79). The mRNA quantification of donkey casein transcripts, carried out by RT-qPCR, showed that the average percentage of corresponding gene transcripts (CSN2, CSN1S1, CSN1S2 I and CSN3) was 70.85, 6.28, 14.23 and 8.65, respectively. The observed translation efficiency, assessed as percentage of single milk casein fraction out of single percentage of transcript, was 0.76, 5.66, 0.50 and 0.32, respectively. The analysis of the sequences flanking the start codon, the codon usage frequencies and the coding sequence length might explain, at least in part, the differential transcriptional and translational rate observed among the casein transcripts.

Keywords

CaseindonkeymRNAquantification
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 201 - 207
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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Footnotes

†

These authors contributed equally to this work.

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