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Identification of novel sources of bacterial leaf blight resistance in wild species of rice

Published online by Cambridge University Press:  05 December 2024

Bhagyasri Tannidi ,
Ishwarya Lakshmi V. G ,
Anantha M.S Open the ORCID record for Anantha M.S [Opens in a new window] ,
G. S. Laha ,
R. M. Sundaram ,
P. Senguttuvel Open the ORCID record for P. Senguttuvel [Opens in a new window] ,
Raveendra Chandavarapu ,
Aleena Dasari ,
Irfan Ahmad Ghazi  and
Padma V
...Show all authors
Bhagyasri Tannidi
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India Acharya N.G. Ranga Agricultural University, Lam, Andhra Pradesh 500030, India
Ishwarya Lakshmi V. G
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
Anantha M.S
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
G. S. Laha
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
R. M. Sundaram
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
P. Senguttuvel
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
Raveendra Chandavarapu
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
Aleena Dasari
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India
Irfan Ahmad Ghazi
Affiliation:
University of Hyderabad, Gachibowli, Hyderabad 500046, India
Padma V
Affiliation:
Acharya N.G. Ranga Agricultural University, Lam, Andhra Pradesh 500030, India
Lakshmi Narayana Reddy Vamireddy
Affiliation:
Acharya N.G. Ranga Agricultural University, Lam, Andhra Pradesh 500030, India
Gireesh Channappa*
Affiliation:
ICAR-Indian Institute of Rice Research, Hyderabad 500030, India ICAR-Indian Institute of Seed Sciences, Regional Station, Bengaluru 560065, India
*
Corresponding author: Gireesh Channappa; Email: [email protected]
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Abstract

Wild species of rice possess tremendous genetic variations and harbour resistance genes for biotic stresses. Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is a major disease affecting rice production globally. The current study characterized 116 accessions from 17 species of Oryza for BB disease during three seasons viz., kharif 2020, rabi 2020–21, kharif 2021 using an isolate of Xanthomonas oryzae pv. oryzae (Xoo) strain IX-020. A total of 40 accessions including Oryza rufipogon, O. nivara, O. officinalis and O. australiensis showed consistence resistance to the bacterial blight disease across the seasons. These accessions were further subjected to molecular characterization using 11 Xa genes viz., Xa4, xa5, xa13, Xa21, Xa23, Xa27(t), Xa32(t), Xa33, Xa35(t), Xa38 and xa41 with gene-specific markers to ascertain the novelty. Some key resistance genes such as Xa4, Xa23, Xa27(t), Xa32(t), Xa33, Xa35(t) and xa41 were detected in multiple accessions, with O. rufipogon and O. eichingeri harbouring particularly complex combinations of these genes. Notably, several accessions viz., IC521672 (O. nivara), EC861665 (O. officinalis), EC861677 (O. latifolia), EC861711 (O. punctata) and EC861738 (O. eichingeri) did not show the presence of any known genes indicating the possibility of novel genetic loci conferring BB resistance in these wild species. These promising accessions identified in the study are potential novel sources for bacterial leaf blight resistance in rice and will be useful for the development of durable bacterial blight resistance rice cultivars.

Keywords

bacterial blightphenotypingSSR markersvalidationwild rice
Type
Research Article
Information
Plant Genetic Resources , Volume 23 , Issue 2 , April 2025 , pp. 129 - 137
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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