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Thirty days of double-strain probiotic supplementation increases monocyte phagocytosis in marathon runners

Published online by Cambridge University Press:  03 June 2024

Edgar Tavares-Silva ,
Geovana Silva Fogaça Leite ,
Helena Batatinha ,
Ayane Resende ,
Valdir de Aquino Lemos Open the ORCID record for Valdir de Aquino Lemos [Opens in a new window] ,
Camila Guazzelli Marques ,
Antônio Herbert Lancha-Jr ,
José Rosa Neto  and
Ronaldo Thomatieli-Santos Open the ORCID record for Ronaldo Thomatieli-Santos [Opens in a new window]
Edgar Tavares-Silva
Affiliation:
Department of Psychobiology, Federal University of São Paulo, São Paulo, SP, Brazil
Geovana Silva Fogaça Leite
Affiliation:
Department of Biodynamics of Human Body Movement, University of São Paulo, São Paulo, SP, Brazil Department of Pediatrics, The University of Arizona, Tucson, AZ, USA
Helena Batatinha
Affiliation:
Department of Cellular and Tissue Biology, University of São Paulo, São Paulo, SP, Brazil School of Nutritional Sciences and Wellness, The University of Arizona, Tucson, AZ, USA
Ayane Resende
Affiliation:
Department of Biodynamics of Human Body Movement, University of São Paulo, São Paulo, SP, Brazil Health Sciences Graduate Program, Federal University of Sergipe, Aracaju, Sergipe, Brazil
Valdir de Aquino Lemos
Affiliation:
Department of Psychobiology, Federal University of São Paulo, São Paulo, SP, Brazil
Camila Guazzelli Marques
Affiliation:
Department of Psychobiology, Federal University of São Paulo, São Paulo, SP, Brazil
Antônio Herbert Lancha-Jr
Affiliation:
Department of Biodynamics of Human Body Movement, University of São Paulo, São Paulo, SP, Brazil
José Rosa Neto
Affiliation:
Department of Cellular and Tissue Biology, University of São Paulo, São Paulo, SP, Brazil
Ronaldo Thomatieli-Santos*
Affiliation:
Department of Psychobiology, Federal University of São Paulo, São Paulo, SP, Brazil Department of Bioscience, Federal University of São Paulo, Santos, SP, Brazil
*
*Corresponding author: Ronaldo Thomatieli-Santos, email [email protected]
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Abstract

Marathon runners, subjected to intense training regimens and prolonged, exhaustive exercises, often experience a compromised immune response. Probiotic supplementation has emerged as a potential remedy to mitigate the impact of prolonged exercise on athletes. Consequently, this study sought to assess the influence of probiotic supplementation on monocyte functionality both before and after the official marathon race. Twenty-seven runners were randomly and double-blindly assigned to two groups: placebo (n 13) and probiotic (PRO) (n 14). Over 30 d, both groups received supplements – placebo sachets containing maltodextrin (5 g/d) and PRO sachets containing 1 × 1010 colony-forming unit Lactobacillus acidophilus and 1 × 1010 colony-forming unit Bifidobacterium bifidum subsp. lactis. Blood samples were collected, and immunological assays, including phagocytosis, hydrogen peroxide production, cytokine levels and monocyte immunophenotyping, were conducted at four different intervals: baseline (start of supplementation/30 d pre-marathon), 24 h-before (1 d pre-marathon), 1 h-after (1 h post-marathon) and 5 d-after (5 d post-marathon). Monocyte populations remained consistent throughout the study. A notable increase in phagocytosis was observed in the PRO group after 30 d of supplementation. Upon lipopolysaccharide stimulation, both PRO and placebo groups exhibited decreased IL-8 production. However, after the marathon race, IL-15 stimulation demonstrated increased levels of 5 d-after, while IL-1-β, IL-8, IL-10, IL-15 and TNF-α varied across different intervals, specifically within the PRO group. Probiotic supplementation notably enhanced the phagocytic capacity of monocytes. However, these effects were not sustained post-marathon.

Keywords

Probiotic supplementationMonocytesPhagocytosisCytokinesStrenuous exercise and marathon
Type
Research Article
Information
British Journal of Nutrition , Volume 132 , Issue 3 , 14 August 2024 , pp. 298 - 308
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Copyright
© The Author(s), 2024. Published by Cambridge University Press on behalf of The Nutrition Society

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