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Linalyl acetate against larvae of Haemonchus spp. and Trichostrongylus spp. that affects ruminants: considerations about the hormetic effect

Published online by Cambridge University Press:  28 October 2024

V.P. Garbin ,
U.Y. Yoshitani  and
M.B. Molento Open the ORCID record for M.B. Molento [Opens in a new window]
V.P. Garbin
Affiliation:
Laboratory of Veterinary Clinical Parasitology, Department of Veterinary Medicine, Federal University of Paraná. R: dos Funcionários, 1540. Curitiba, Paraná, Brazil. CEP: 80.035-050
U.Y. Yoshitani
Affiliation:
Laboratory of Veterinary Clinical Parasitology, Department of Veterinary Medicine, Federal University of Paraná. R: dos Funcionários, 1540. Curitiba, Paraná, Brazil. CEP: 80.035-050
M.B. Molento*
Affiliation:
Laboratory of Veterinary Clinical Parasitology, Department of Veterinary Medicine, Federal University of Paraná. R: dos Funcionários, 1540. Curitiba, Paraná, Brazil. CEP: 80.035-050
*
Corresponding author: M.B. Molento; Email: [email protected]
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Abstract

Active components from plants are an alternative therapy to parasite control, addressing the widespread multidrug resistance populations. Linalyl acetate (LA), an ester abundantly found in plants of the genus Lavandula, was tested in vitro against third-stage larvae (L3) of Haemonchus spp. and Trichostrongylus spp. using the larval migration test at 0.89, 2.24, 4.47, 8.95, 17.9, 35.8, 71.6, and 143.2 mg/ml. After an initial incubation of 18 h, the total content of each tube was transferred to a 24-well plate that allowed active L3 to migrate through a nylon mesh (second incubation). Although LA exhibited 100% efficacy in reducing larval migration at 8.95 and 17.9 mg/ml, it showed reduced activity (5%) at 143.2 mg/ml. The data revealed a hormetic biphasic response characterised by an inverted U-shaped concentration-response curve. While hormesis has been previously documented in insecticidal and allelopathic contexts, this study reports the occurrence of hormesis induced by a phytochemical component against two species of nematodes for the first time. This distinctive stimulation-and-inhibition effect should be considered when selecting new compounds for preclinical testing.

Keywords

Phytotherapyendoparasitessmall ruminantshormesisdrug resistance
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e59
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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