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Vitrification of bovine embryos followed by in vitro hatching and expansion

Published online by Cambridge University Press:  18 December 2017

J.F. Souza ,
C.M. Oliveira ,
L.L. Lienou ,
T.V. Cavalcante ,
E. Alexandrino ,
R.R. Santos ,
A.P.R. Rodrigues ,
C.C. Campello ,
J.R. Figueiredo  and
F.E.F. Dias
J.F. Souza
Affiliation:
Laboratory Brio Genetics and Biotechnology Ltd, Araguaína, TO, Brazil.
C.M. Oliveira
Affiliation:
Veterinary Medical, Redenção, PA, Brazil.
L.L. Lienou
Affiliation:
Laboratory Brio Genetics and Biotechnology Ltd, Araguaína, TO, Brazil.
T.V. Cavalcante
Affiliation:
Federal University of Piauí, Teresina, PI, Brazil.
E. Alexandrino
Affiliation:
Federal University of Tocantins, EMVZ, Campus Araguaína, TO, Brazil.
R.R. Santos
Affiliation:
Federal University of Pará, Castanhal, PA, Brazil.
A.P.R. Rodrigues
Affiliation:
State University of Ceará, Fortaleza, CE, Brazil.
C.C. Campello
Affiliation:
State University of Ceará, Fortaleza, CE, Brazil.
J.R. Figueiredo*
Affiliation:
Faculdade de Veterinária, Universidade Estadual do Ceará, Av. Silas Munguba, 1700 Campus do Itaperi, 60714–903, Fortaleza, Ceará, Brazil. State University of Ceará, Fortaleza, CE, Brazil.
F.E.F. Dias
Affiliation:
Federal University of Tocantins, EMVZ, Campus Araguaína, TO, Brazil.
*
All correspondence to: J. R. Figueiredo. Faculdade de Veterinária, Universidade Estadual do Ceará, Av. Silas Munguba, 1700 Campus do Itaperi, 60714–903, Fortaleza, Ceará, Brazil. Tel: +55 85 31019858. Fax: +55 85 31019840. E-mail: [email protected]
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Summary

The objective of this study was to assess the effects of bovine embryo vitrification by applying three different vitrification solutions containing ethylene glycol (EG) and dimethylsulphoxide (DMSO) at different concentrations (10, 20 or 25% each) combined with 1.0 M glucose or 1.0 M sucrose, on the in vitro hatching and expansion rates. Healthy oocytes were selected for in vitro maturation and fertilization from 200 bovine ovaries, and subsequently cultured up to the blastocyst stage (n = 800). Control (n = 200) and vitrified cells (n = 100 per treatment; 600 in total) were cultured for an extra 24 or 48 h to evaluate hatching and expansion, respectively. Vitrification significantly decreased embryonic re-expansion and hatching rates independently of the tested solution when compared with control embryos, but solutions with 25% EG + 25% DMSO resulted in the highest re-expansion (75%) and hatching (70%) rates, independently of the added sugar. The addition of sucrose resulted in higher rates of re-expanded and hatched embryos when compared with glucose addition. We concluded that the combination of 25% EG + 25% DMSO and 1.0 M sucrose allowed hatching and expansion of vitrified-warmed bovine embryos produced in vitro.

Keywords

DMSOEGEmbryo vitrificationGlucoseSucrose
Type
Short Communication
Information
Zygote , Volume 26 , Issue 1 , February 2018 , pp. 99 - 103
Copyright
Copyright © Cambridge University Press 2017 

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