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Ultrastructural changes of sheep cumulus–oocyte complexes following different methods of vitrification

Published online by Cambridge University Press:  17 February 2011

Bita Ebrahimi ,
Mojtaba Rezazadeh Valojerdi ,
Poopak Eftekhari-Yazdi  and
Hossein Baharvand
Bita Ebrahimi
Affiliation:
Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, P.O. Box: 14115–111, Tehran, Iran.
Mojtaba Rezazadeh Valojerdi*
Affiliation:
Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, P.O. Box: 14115–111, Tehran, Iran. Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, Tehran, Iran.
Poopak Eftekhari-Yazdi
Affiliation:
Department of Embryology, Royan Institute for Reproductive Biomedicine Research, ACECR, Tehran, Iran.
Hossein Baharvand
Affiliation:
Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran.
*
All correspondence to: Mojtaba Rezazadeh Valojerdi. Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, P.O. Box: 14115–111, Tehran, Iran. Tel: +9821 82883897. Fax: +9821 88013030. e-mail: [email protected] or [email protected]
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Summary

To determine the ultrastructural changes of sheep cumulus–oocyte complexes (COCs) following different methods of vitrification, good quality isolated COCs (GV stage) were randomly divided into the non-vitrified control, conventional straw, cryotop and solid surface vitrification groups. In both conventional and cryotop methods, vitrified COCs were respectively loaded by conventional straws and cryotops, and then plunged directly into liquid nitrogen (LN2); whereas in the solid surface group, vitrified COCs were first loaded by cryotops and then cooled before plunging into LN2. Post-warming survivability and ultrastructural changes of healthy COCs in the cryotop group especially in comparison with the conventional group revealed better viability rate and good preservation of the ooplasm organization. However in all vitrification groups except the cryotop group, mitochondria were clumped. Solely in the conventional straw group, the mitochondria showed different densities and were extremely distended. Moreover in the latter group, plenty of large irregular connected vesicles in the ooplasm were observed and in some parts their membrane ruptured. Also, in the conventional and solid surface vitrification groups, cumulus cells projections became retracted from the zona pellucida in some parts. In conclusion, the cryotop vitrification method as compared with other methods seems to have a good post-warming survivability and shows less deleterious effects on the ultrastructure of healthy vitrified–warmed sheep COCs.

Keywords

Cumulus–oocyte complexesSheepUltrastructureVitrification
Type
Research Article
Information
Zygote , Volume 20 , Issue 2 , May 2012 , pp. 103 - 115
Copyright
Copyright © Cambridge University Press 2011

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References

Arav, A., Shehu, D. & Mattioli, M. (1993). Osmotic and cytotoxic study of vitrification of immature bovine oocytes. J. Reprod. Fertil. 99, 353–8.CrossRefGoogle ScholarPubMed
Asada, M., Ishibashi, S., Ikumi, S. & Fukui, Y. (2002). Effect of polyvinyl alcohol (PVA) concentration during vitrification of in vitro matured bovine oocytes. Theriogenology 58, 1199–208.CrossRefGoogle ScholarPubMed
Bogliolo, L., Ariu, F., Fois, S. et al. (2007). Morphological and biochemical analysis of immature ovine oocytes vitrified with or without cumulus cells. Theriogenology 68, 1138–49.CrossRefGoogle ScholarPubMed
Chen, S.U., Lien, Y.R., Chen, H.F., et al. (2001). Vitrification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws (OPS) and grids. Hum. Reprod. 16, 2350–6.CrossRefGoogle ScholarPubMed
Combelles, C.M., Cekleniak, N.A., Racowsky, C. & Albertini, D.F. (2002). Assessment of nuclear and cytoplasmic maturation in vitro. Hum. Reprod. 17, 1006–16.CrossRefGoogle Scholar
Coticchio, G., Borini, A., Distratis, V. et al. (2010). Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols. J. Assist. Reprod. Genet. 27, 131–40.CrossRefGoogle ScholarPubMed
Cran, D.G., Hay, M. & Hay, M.F. (1980). Fine structure of the sheep oocytes during antral follicle development. J. Reprod. Fertil. 59, 125–32.CrossRefGoogle ScholarPubMed
Diez, C., Duque, P., Gomez, E. et al. (2005). Bovine oocyte vitrification before or after meiotic arrest: effect on ultrastructure and developmental ability. Theriogenology 64, 317–333.CrossRefGoogle ScholarPubMed
Dumollard, R., Duchen, M. & Carroll, J. (2007). The role of mitochondrial function in the oocyte and embryo. Curr. Top. Dev. Biol. 77, 21–49.CrossRefGoogle ScholarPubMed
Ebner, T., Moser, M., Sommergruber, M. et al. (2005). Occurrence and developmental consequences of vacuoles throughout preimplantation development. Fertil. Steril. 83, 1635–40.CrossRefGoogle ScholarPubMed
Ebrahimi, B., Valojerdi, M.R., Yazdi, P.E. et al. (2010). IVM and gene expression of sheep cumulus–oocyte complexes following vitrification by different methods. Reprod. Biomed. Online 20, 26–34.CrossRefGoogle ScholarPubMed
Eroglu, A., Toner, M., Leykin, L. & Toth, T.L. (1998). Cytoskeleton and polyploidy after maturation and fertilization of cryopreserved germinal vesicle stage mouse oocytes. J. Assist. Reprod. Genet. 15, 447–54.CrossRefGoogle ScholarPubMed
Fleming, W.N. & Saake, R.G. (1972). Fine structure of the bovine oocytes from the mature Graafian follicle. J. Reprod. Fertil. 29, 203–13.CrossRefGoogle ScholarPubMed
Fu, X.W., Shi, W.Q., Zhang, Q.J. et al. (2009). Positive effects of taxol pretreatment on morphology, distribution and ultrastructure of mitochondria and lipid droplets in vitrification of in vitro matured porcine oocytes. Anim. Reprod. Sci. 115, 158–68CrossRefGoogle ScholarPubMed
Fuku, E., Liu, J. & Downey, B.R. (1995a). In vitro viability and ultrastructural changes in bovine oocytes treated with a vitrification solution. Mol. Reprod. Dev. 40, 177–85.CrossRefGoogle ScholarPubMed
Fuku, E., Xia, L. & Downey, B.R. (1995b). Ultrastructural changes in bovine oocytes cryopreserved by vitrification. Cryobiology 32, 139–56.CrossRefGoogle ScholarPubMed
Gilchrist, R.B., Ritter, L.J. & Armstrong, D.T. (2004). Oocyte–somatic cell interaction during follicle development in mammals. Anim. Reprod. Sci. 82, 431–46.CrossRefGoogle ScholarPubMed
Ghadially, F.N. (1982). Ultrastructural Pathology of the Cell and Matrix, 2nd edn. Butterworths, London, UK.Google Scholar
Ghelter, Y., Skutelsky, E., Nun, I.B., et al. (2006). Human oocyte cryopreservation and the fate of cortical granules. Fertil. Steril. 86, 210–6.Google Scholar
Goud, A., Goud, P., Qian, C., et al. (2000). Cryopreservation of human germinal vesicle stage and in vitro matured MII oocytes: influence of cryopreservation media on the survival, fertilization and early cleavage divisions. Fertil. Steril. 74, 487–94.CrossRefGoogle Scholar
Grondahl, C., Hyttel, P., Grondahl, M.L., et al. (1995). Structural and endocrine aspects of equine oocyte maturation in vivo. Mol. Reprod. Dev. 42, 94–105.CrossRefGoogle ScholarPubMed
Gualtieri, R., Iaccarino, M., Mollo, V., et al. (2009). Slow cooling of human oocytes: ultrastructural injuries and apoptotic status. Fertil. Steril. 91, 1023–1034.CrossRefGoogle ScholarPubMed
Hochi, S., Kozawa, M., Fujimoto, T., et al. (1996). In vitro maturation and transmission electron microscopic observation of horse oocytes after vitrification. Cryobiology 33, 300–10.CrossRefGoogle ScholarPubMed
Hyttel, P., Xu, K.P., Smith, S. & Greve, T. (1986). Ultrastructure of in-vitro oocyte maturation in cattle. J. Reprod. Fertil. 78, 615–25.CrossRefGoogle ScholarPubMed
Hyttel, P., Greve, T. & Callesen, H. (1989). Ultrastructural aspects of oocyte maturation and fertilization in cattle. J. Reprod. Fertil. Suppl. 38, 35–47.Google ScholarPubMed
Hyttel, P., Vajta, G. & Callesen, H. (2000). Vitrification of bovine oocytes with the open pulled straw method: ultrastructure consequences. Mol. Reprod. Dev. 56, 80–8.3.0.CO;2-U>CrossRefGoogle ScholarPubMed
Isachenko, V., Isachenko, E., Michelmann, H.W. et al. (2001). Lipolysis and ultrastructure changes of intracellular lipid vacuoles after cooling of bovine and porcine GV-oocytes. Anat. Histol. Embryol. 30, 333–8.CrossRefGoogle Scholar
Isachenko, V., Selman, H., Isachenko, E. et al. (2003). Modified vitrification of human pronuclear oocytes: efficacy and effect on ultrastructure. Reprod. Biomed. Online 7, 211–6.CrossRefGoogle ScholarPubMed
Isachenko, V., Montag, M., Isachenko, E. et al. (2004). Developmental rate and ultrastructure of vitrified human pronuclear oocytes after step-wise versus direct rehydration. Hum. Reprod. 19, 660–5.CrossRefGoogle ScholarPubMed
Johnson, M.H., Pickering, S.J. & George, M.A. (1988). The influence of cooling on the properties of the zona pellucida of the mouse oocyte. Hum. Reprod. 9, 383–7.CrossRefGoogle Scholar
Kline, D. & Kline, J. (1992). Repetitive calcium transients and the role of calcium in exocytosis and cell cycle activation in the mouse egg. Dev. Biol. 149, 80–9.CrossRefGoogle ScholarPubMed
Kuwayama, M. & Kato, O. (2000). All-around vitrification method for human oocytes and embryos. J. Assist. Reprod. Genet. 17, 477.Google Scholar
Li, G.P., Bunch, T.D., White, K.L. et al. (2006). Denuding and centrifugation of maturing bovine oocytes alters oocyte spindle integrity and viability of cytoplasm to support parthenogenetic and nuclear transfer embryo development. Mol. Reprod. Dev. 73, 446–451.CrossRefGoogle Scholar
Makabe, S., Van Blerkom, J., Nottola, S.A. et al. (2006). Atlas of Human Female Reproductive Function. Ovarian Development to Early Embryogenesis after In-vitro Fertilization. Taylor and Francis, London.Google Scholar
Motta, P.M., Nottola, S.A., Pereda, J. et al. (1995). Ultrastructure of human cumulus oophorus: a transmission electron microscopic study on oviductal oocytes and fertilized eggs. Hum. Reprod. 10, 2361–2367.CrossRefGoogle ScholarPubMed
Motta, P.M., Nottola, S.A., Makabe, S. & Heyn, R. (2000). Mitochondrial morphology in human fetal and adult female germ cells. Hum. Reprod. 15 (Suppl 2), 129–147.CrossRefGoogle ScholarPubMed
Motta, P.M., Nottola, S.A., Familiari, G. et al. (2003). Morphodynamics of the follicular–luteal complex during early ovarian development and reproductive life. Int. Rev. Cytol. 223, 177–288.CrossRefGoogle ScholarPubMed
Nottola, S.A., Coticchio, G., Sciajno, R. et al. (2009). Ultrastructural markers of quality in human mature oocytes vitrified using cryoleaf and cryoloop. Reprod. Biomed. Online 19, 17–27.CrossRefGoogle ScholarPubMed
Park, S.E., Son, W.Y., Lee, S.H. et al. (1997). Chromosome and spindle configurations of human oocytes matured in vitro after cryopreservation at the germinal vesicle stage. Fertil. Steril. 68, 920–6.CrossRefGoogle ScholarPubMed
Pickering, S.J., Braude, P.R., Johnson, M.H. et al. (1990). Transient cooling to room temperature can cause irreversible disruption of meiotic spindle in the human oocyte. Fertil. Steril. 54, 102–8.CrossRefGoogle ScholarPubMed
Reader, L.K. (2007). A Quantitative Ultrastructural Study of Oocytes during the Early Stages of Ovarian Follicular Development in Booroola and Wild-type Sheep. A thesis of Victoria University of Wellington.Google Scholar
Rho, G.J., Kim, S., Yoo, J.G., et al. (2002). Microtubulin configuration and mitochondrial distribution after ultra-rapid cooling of bovine oocytes. Mol. Reprod. Dev. 63, 464–70.CrossRefGoogle ScholarPubMed
Ruppert-Lingham, C.J., Paynter, S.J., Godfrey, J. et al. (2003). Developmental potential of murine germinal vesicle stage cumulus–oocyte complexes following exposure to dimethylsulphoxide or cryopreservation: loss of membrane integrity of cumulus cells after thawing. Hum. Reprod. 18, 392–8.CrossRefGoogle ScholarPubMed
Russe, I. (1975). Unfertilized sheep eggs. J. Reprod. Med. 14, 200–4.Google ScholarPubMed
Schalkoff, M., Oskowitz, S. & Douglas Powers, R. (1989). Ultrastructural observations of human and mouse oocytes treated with cryopreservatives. Biol. Reprod. 40, 379–93.CrossRefGoogle ScholarPubMed
Shi, L.Y., Jin, H.F., Kim, J.G. et al. (2007). Ultrastructural changes and development potential of porcine oocytes following vitrification. Anim. Reprod. Sci. 100, 128–40.CrossRefGoogle ScholarPubMed
Swaine, J., Pool, T.B. (2008). ART failure: oocyte contributions to unsuccessful fertilization. Hum. Reprod. Update 14, 431–46.CrossRefGoogle Scholar
Tucker, M.J., Morton, P.C., Wright, G. & Massey, J.B. (1998). Birth after cryopreservation of immature oocytes with subsequent in vitro maturation. Fertil. Steril. 70, 578–9.CrossRefGoogle ScholarPubMed
Vajta, G. (1997). Featured article: vitrification of bovine oocytes and embryos. Embryo Transfer Newsletter 15, 12–8.Google Scholar
Valojerdi, M.R. & Salehnia, M. (2005). Developmental potential and Ultrastructural injuries of metaphase II (MII) mouse oocytes after slow freezing or vitrification. J. Assist. Reprod. Genet. 22, 119–27.CrossRefGoogle ScholarPubMed
Van Blerkom, J. (1990). Occurrence and developmental consequences of aberrant cellular organization in meiotically mature human oocytes after exogenous ovarian hyperstimulation. J. Electron. Microsc. Tech. 16, 324–46.CrossRefGoogle ScholarPubMed
Vincent, C., Pickering, S.J., Jhonson, M.H. & Quick, S.J. (1990). Dimethyl sulphoxide affects the organization of microfilaments in the mouse oocytes. Mol. Reprod. Dev. 26, 227–35.CrossRefGoogle Scholar
Whitaker, M. (2006). Calcium at fertilization and in early development. Physiol. Rev. 86, 25–88.CrossRefGoogle ScholarPubMed
Wongsrikeao, P., Kanashige, Y., Ooki, R. et al. (2005). Effect of removal of cumulus cells on the nuclear maturation, fertilization and development of porcine oocytes. Reprod. Dom. Anim. 40, 166–70.CrossRefGoogle ScholarPubMed
Yang, Q.Z., Sun, Q.Y., Liu, G.Y. et al. (1994). Developmental competence and ultrastructure damage of cryopreserved GV-stage bovine oocytes. Theriogenology 41, 342 (Abstract).CrossRefGoogle Scholar
Younis, A.I., Toner, M., Albertini, D.F. & Bigger, J.D. (1996). Cryobiology of non-human oocytes. Hum. Reprod. 11, 156–65.CrossRefGoogle ScholarPubMed