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Traditional intracytoplasmic sperm injection provides equivalent outcomes compared with human zona pellucida-bound selected sperm injection

Published online by Cambridge University Press:  09 May 2013

Valentina Casciani ,
Maria Giulia Minasi ,
Gemma Fabozzi ,
Filomena Scarselli ,
Alessandro Colasante ,
Anna Maria Lobascio  and
Ermanno Greco
Valentina Casciani
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Maria Giulia Minasi
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Gemma Fabozzi*
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Filomena Scarselli
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Alessandro Colasante
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Anna Maria Lobascio
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
Ermanno Greco
Affiliation:
Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy.
*
All correspondence to: Gemma Fabozzi. Center for Reproductive Medicine, European Hospital, Via Portuense 700 – 00149 Rome, Italy. Tel: +39 06 65975659. fax: +39 06 6534897. e-mail: [email protected]
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Summary

The capability of human zona pellucida (ZP) to bind selectively to normal functional sperm with normal chromatin has been reported widely in the literature. The aim of this study was to evaluate whether ZP–binding sperm selection may represent a method to retrieve superior spermatozoa for intracytoplasmic sperm injection (ICSI). Patients were divided into two groups: a ZP-ICSI and a conventional ICSI group. In the ZP-ICSI group, spermatozoa for injection were selected after ZP–sperm incubation and spermatozoa that were tightly bound to the ZP were used for ICSI (ZP-ICSI). Clinical outcomes of ZP-ICSI were compared with the outcomes of traditional scientist-selected sperm injection (conventional ICSI). Results did not show any significant difference in fertilization, pregnancy, implantation and take-home-baby rates between conventional ICSI and ZP-ICSI. However, when data relative to patients who received ZP-ICSI were analyzed, an interesting result was observed: higher sperm concentration and morphology correlated with higher ZP–sperm binding. Additionally, patients with higher ZP–sperm binding seem to have improved pregnancy and take-home-baby rates. In conclusion, this study shows that ZP-ICSI is not a superior method compared with conventional ICSI. However, clinical ICSI outcomes were apparently improved in the presence of good ZP–sperm binding. We therefore speculate that sperm competence to ICSI could be reduced when the sperm's ability to bind the ZP is impaired.

Keywords

Sperm selectionZona pellucidaZP-ICSIZP–sperm binding
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 565 - 570
Copyright
Copyright © Cambridge University Press 2013 

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References

Agarwal, A., Juris, E. & Rakesh, S. (2009). Sperm chromatin assessment. In Textbook of Assisted Reproductive Technologies, (eds Gardner, D., Weissman, A., Howles, C.M. & Shoham, Z.) pp. 67–84. London, United Kingdom: Informa Healthcare.Google Scholar
Aitken, R.J. & Krausz, C. (2001). Oxidative stress, DNA damage and the Y chromosome. Reproduction 122, 497–506.Google Scholar
Benchaib, M., Lornage, J., Mazoyer, C., Lejeune, H., Salle, B. & François Guerin, J. (2007). Sperm deoxyribonucleic acid fragmentation as a prognostic indicator of assisted reproductive technology outcome. Fertil. Steril. 87, 93–100.CrossRefGoogle ScholarPubMed
Black, M., Liu, D.Y., Bourne, H. & Backer, H.W.G. (2010). Comparison of outcomes of conventional intracytoplasmic sperm injection and intracytoplasmic sperm injection using sperm bound to the zona pellucida of immature oocytes. Fertil. Steril. 93, 6724.CrossRefGoogle Scholar
Garrett, C., Liu, D.Y. & Baker, H.W.G. (1997). Selectivity of the human sperm–zona pellucida binding process to sperm head morphometry. Fertil. Steril. 67, 362–71.CrossRefGoogle ScholarPubMed
Garrett, C., Liu, D.Y., Clarke, G.N., Rushford, D.D. & Baker, H.W.G. (2003). Automated semen analysis: ‘zona pellucida preferred’ sperm morphology and straight-line velocity are related to pregnancy rate in subfertile couples. Hum. Reprod. 18, 1643–9.Google Scholar
Kheirollahi-Kouhestani, M., Razavi, S., Tavalaee, M., Deemeh, M.R., Mardani, M., Moshtaghian, J. & Nasr-Esfahani, M.H. (2009). Selection of sperm based on combined density gradient and zeta method may improve ICSI outcome. Hum. Reprod. 24, 2409–16.CrossRefGoogle ScholarPubMed
Liu, D.Y. & Baker, H.W.G. (1994). Acrosome status and morphology of human sperm bound to the zona pellucida and oolemma determined using oocytes that failed to fertilize in vitro. Hum. Reprod. 9, 673–9.Google Scholar
Liu, D.Y. & Baker, H.W.G. (2002). Evaluation and assessment of semen for IVF/ICSI. Asian J. Androl. 4, 281–5.Google ScholarPubMed
Liu, D.Y. & Baker, H.W.G. (2004). High frequency of defective sperm–zona pellucida interaction in oligozoospermic infertile men. Hum. Reprod. 19, 228–33.Google Scholar
Liu, D.Y. & Baker, H.W.G. (2007). Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence. Hum. Reprod. 22, 1597–602.Google Scholar
Liu, D.Y., Garrett, C. & Baker, H.W.G. (2003). Low proportion of sperm can bind to the zona pellucida of human oocytes. Hum. Reprod. 18, 2382–9.Google Scholar
Liu, D.Y., Liu, M.L. & Baker, H.W.G. (2009). Enhancement of sperm–zona pellucida (ZP) binding capacity by activation of protein kinase A and C pathways in certain infertile men with defective sperm–ZP binding. Hum. Reprod. 24, 20–7.Google Scholar
Liu, F., Qiu, Y., Zou, Y., Deng, Z.H., Yang, H. & Liu, D.Y. (2011). Use of zona pellucida-bound sperm for intracytoplasmic sperm injection produces higher embryo quality and implantation than conventional intracytoplasmic sperm injection. Fertil. Steril. 95, 815–81.Google Scholar
Lopata, A. & Leung, P.C. (1988). The fertilizability of human oocytes at different stage of meiotic maturation. In In Vitro Fertilization and Other Assisted Reproduction (eds Jones, H.W. Jr & Schrader, C.) pp. 324–36. New York: New York Academy of Science.Google Scholar
Menkveld, R., Franken, D.R., Kruger, T.F., Oehninger, S. & Hodgen, G.D. (1991). Sperm selection capacity of the human zona pellucida.Mol. Reprod. Dev. 30, 346–52.CrossRefGoogle ScholarPubMed
Oehninger, S., Mahony, M., Ozgur, K., Kolm, P., Kruger, T. & Franken, D. (1997). Clinical significance of human sperm–zona pellucida binding. Fertil. Steril. 67, 1121–7.CrossRefGoogle ScholarPubMed
Overstreet, J.W. & Hembree, W.C. (1976). Penetration of the zona pellucida of non living human oocytes by human spermatozoa in vitro. Fertil. Steril. 27, 815–31.CrossRefGoogle Scholar
Ozmen, B., Koutlaki, N., Youssry, M., Diedrich, K. & Al-Hasani, S. (2007). DNA damage of human spermatozoa in assisted reproduction: origins, diagnosis, impacts and safety. Reprod. Biomed. Online 14, 384–95.Google Scholar
Paes Almeida Ferreira de Braga, D., Iaconelli, A. Jr, Cássia Sávio de Figueira, R., Madaschi, C., Semião-Francisco, L. & Borges, E. Jr (2009). Outcome of ICSI using zona pellucida-bound spermatozoa and conventionally selected spermatozoa. Reprod. Biomed. Online 19, 802–7.Google ScholarPubMed
Razavi, S., Nasr-Esfahani, M.H., Mardani, M., Mafi, A. & Moghdam, A. (2003). Effect of human sperm chromatin anomalies on fertilization outcome post-ICSI. Andrologia 35, 238–43.CrossRefGoogle ScholarPubMed
Rienzi, L., Ubaldi, F.M., Iacobelli, M., Ferrero, S., Minasi, M.G., Martinez, F., Tesarik, J. & Greco, E. (2002). Day 3 embryo transfer with combined evaluation at the pronuclear and cleavage stages compares favourably with day 5 blastocyst transfer. Hum. Reprod. 17, 1852–5.Google Scholar
Rienzi, L., Ubaldi, F.M., Iacobelli, M., Minasi, M.G., Romano, S., Ferrero, S., Sapienza, F., Baroni, E., Litwicka, K. & Greco, E. (2008). Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil. Steril. 90, 1692–700.CrossRefGoogle ScholarPubMed
c, N., Nadalini, M., Bizzaro, D., Serrao, L., Fava, L., Scaravelli, G. & Borini, A. (2009). Sperm-hyaluronan-binding assay: clinical value in conventional IVF under Italian law. Reprod. Biomed. Online 19, 35–43.Google Scholar
Tavalaee, M., Razavi, S. & Nasr-Esfahani, M.H. (2009). Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil. Steril. 91, 1119–26.CrossRefGoogle ScholarPubMed
Tesarik, J. (2005). Paternal effects on cell division in the human preimplantation embryo. Reprod. Biomed. Online 10, 370–5.Google Scholar
Tesarik, J., Mendoza, C. & Greco, E. (2002). Paternal effect acting during the first cell cycle of human preimplantation development after ICSI. Hum. Reprod. 17, 184–189.CrossRefGoogle Scholar
Tomlinson, M.J., Moffatt, O., Manicardi, G.C., Bizzarro, D., Afnan, M. & Sakkas, D. (2001). Interrelationship between seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception. Hum. Reprod. 16, 2160–5.Google Scholar
World Health Organization (2009). WHO Laboratory Manual for the Examination of Human Semen and Sperm–Cervical Mucus Interaction, 4th edition. Cambridge University Press, Cambridge, UK.Google Scholar