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Synergetic effects of epidermal growth factor and gonadotropins on the cytoplasmic maturation of pig oocytes in a serum–free medium

Published online by Cambridge University Press:  26 September 2008

Weihua Wanga  and
Koji Niwa
Weihua Wanga*
Affiliation:
Okayam University, Okayama 700, Japan.
Koji Niwa
Affiliation:
Okayam University, Okayama 700, Japan.
*
Weihua Wang, Laboratory of Embryology, Department of Reproduction, National Institute of Animal Industry, Tsukuba Norindanchi PO Box 5, Ibaraki, 305 Japan. Telephone: 0298-38-8636. Fax: 0298-38-8606.
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Summary

Cumulus-enclosed or cumulus-free pig oocytes were cultured for 48 h in a serum-free maturation medium, TCM-199B containing 1 mg/ml polyvinylalcohol, supplemented with gonadotropins (PMSG + hCG) and/or epidermal growth factor (EGF), and inseminated with frozen-thawed ejaculated spermatozoa. Fourteen hours after insemination, the ability of oocytes to form male pronucleus (MPN) was examined. EGF significantly increased the ability of cumulus-enclosed oocytes to form MPN irrespective of the concentrations (1–100 ng/ml) tested. Gonadotropins had a greater effect than EGF on MPN formation in cumulus-enclosed oocytes, and a synergetic effect was observed when EGF and gonadotropins were added together. However, neither EGF nor gonadotropins exhibited a stimulatory effect on MPN formation in cumulus-free oocytes. These results indicate that the role(s) of both EGF and gonadotropins in stimulating cytoplasmic maturation of pig oocytes is mediated by cumulus.

Keywords

EGFGonadotropinOocyte cytoplasmic maturationPigSerum-free medium
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 345 - 350
Copyright
Copyright © Cambridge University Press 1995

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References

Coskun, S., Sanbuissho, A., Lin, Y.C. & Rikihisa, Y. (1991). Fertilizability and subsequent developmental ability of bovine oocytes matured in medium containing epidermal growth factor (EGF). Theriogenology 36, 485–94.CrossRefGoogle ScholarPubMed
Das, K., Tagatz, G.E., Stout, L.E., Phipps, W.R., Hensleigh, H.C.. & Leung, B.S. (1991). Direct positive effect of epidermal growth factor on the cytoplasmic maturation of mouse and human oocytes. Fertil. Steril. 55, 1000–4.CrossRefGoogle ScholarPubMed
Ding, J.C.. & Foxcroft, G.R. (1994). Epidermal growth factor enhances oocyte maturation in pigs. Mol. Reprod. Dev. 39, 30–40.CrossRefGoogle ScholarPubMed
Downs, S.M., Schroeder, A.C.. & Eppig, J.J. (1986). Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing hardening of the zona pellucida. Gamete Res. 15, 115–22.CrossRefGoogle Scholar
Funahashi, H.. & Day, B.N. (1993). Effects of different serum supplements in maturation medium on meiotic and cytoplasmic maturation of pig oocytes. Theriogenology 39, 965–73.CrossRefGoogle ScholarPubMed
Funahashi, H., Cantley, T. & Day, B.N. (1994). Different hormonal requirements of pig oocyte-cumulus complexes during maturation in vitro. J. Reprod. Fertil. 101, 159–65.CrossRefGoogle ScholarPubMed
Harper, K.M.. & Brackett, B.G. (1993). Bovine blastocyst development after in vitro maturation in a defined medium with epidermal growth factor and low concentrations of gonadotropins. Biol. Reprod. 48, 409–16.CrossRefGoogle Scholar
Hsu, C.J., Holmes, S.D. & Hammond, J.M. (1987). Ovarian epidermal growth factor-like activity concentrations in porcine follicular fluid during follicular enlargement. Biochem. Bioiphys. Res. Commun. 147, 242–7.CrossRefGoogle ScholarPubMed
Kikuchi, K., Nagai, T., Motlik, J., Shioya, Y. & Izaike, Y. (1993). Effect of follicle cells on in vitro fertilization of pig follicular oocytes. Theriogenology 39, 593–9.CrossRefGoogle ScholarPubMed
Kobayashi, K., Yamashida, S. & Hoshi, H. (1994). Influence of epidermal growth factor and transforming growth factor-a on in vitro maturation of cumulus-enclosed bovine oocytes in a defined medium. J. Reprod. Fertil. 100, 439–46.CrossRefGoogle Scholar
Mattioli, M., Galeati, G. & Seren, E. (1988 a). Effect of follicle somatic cells during pig oocyte maturation on egg penetrability and male pronucleus formation. Gamete Res. 20, 177–83.CrossRefGoogle ScholarPubMed
Mattioli, M., Galeati, J., Bacci, M.L. & Seren, E. (1988 b). Follicular factors influence oocyte fertilizability by modulating the intercellular cooperation between cumulus cell and oocyte. Gamete Res. 21, 223–32.CrossRefGoogle ScholarPubMed
Mattioli, M., Bacci, M.L., Galeati, G. & Seren, E. (1991). Effects of LH and FSH on the maturation of pig oocytes in vitro. Theriogenology 36, 95–105.CrossRefGoogle ScholarPubMed
Naito, K., Fukuda, Y. & Toyoda, Y. (1988). Effect of porcine follicular fluid on male pronucleus formation in porcine oocytes matured in vitro. Gamete Res. 21, 289–95.CrossRefGoogle ScholarPubMed
Reed, M.L., Estrada, J.L., Illera, M.J.. & Petters, R.M. (1993). Effects of epidermal growth factor, insulin-like growth factor-I, and dialyzed porcine follicular fluid on porcine oocytes maturation in vitro. J. Exp. Zool. 266, 74–8.CrossRefGoogle ScholarPubMed
Vanderhyden, B.C. & Armstrong, D.T. (1989). Role of cumulus cells and serum on, the in vitro maturation, fertilization, and subsequent development of rat oocytes. Biol. Reprod. 40, 720–8.CrossRefGoogle ScholarPubMed
Wang, W.H. & Niwa, K. (1995). Effects of epidermal growth factor (EGF) and gonadotropins on nuclear maturation and cumulus expansion of pig oocytes in a serum-free medium. Assist. Reprod. Tech. Androl. 7, 35–46.Google Scholar
Wang, W.H., Abeydeera, L.R., okuda, K. & Niwa, K. (1994). Penetration in vitro of porcine follicular oocytes during maturation by cryopreserved, ejaculated spermatozoa. Biol. Reprod. 50, 510–15.CrossRefGoogle Scholar
Yoshida, M., Bamba, K. & Kojima, Y. (1989). Effects of gonadotropins and estradiol 17β on the timing of nuclear maturation and cumulus mass expansion in pig oocytes cultured in vitro. Jpn. J. Anim. Reprod. 35, 86–91.CrossRefGoogle Scholar
Yoshida, M., Ishigaki, Y., Kawagishi, H., Bamba, K.. & Kojima, Y. (1992). Effects of pig follicular fluid on maturation of pig oocytes in vitro and on their subsequent fertilizing and developmental capacity in vitro. J. Reprod. Fertil. 95, 481–8.CrossRefGoogle ScholarPubMed
Yoshida, M., Ishigaki, K., Nagai, T., Chikyu, M. & Pursel, V.G. (1993). Glutathione concentration during maturation and after fertilization in pig oocyte: relevance to the ability of oocytes to form male pronucleus. Biol. Reprod. 49, 89–94.CrossRefGoogle Scholar
Younis, A.I., Brackett, B.G.. & Feyrer-Hosken, R.A. (1989). Influence of serum and hormone on bovine oocyte maturation and fertilization in vitro. Gamete Res. 23, 189–201.CrossRefGoogle ScholarPubMed
Zheng, Y.S., Fiser, P.. & Sirard, M.A. (1992). The use of ejaculated boar semen after freezing in 2 and 6% glycerol for in vitro fertilization of porcine oocytes matured in vitro. Theriogenology 38, 1065–75.CrossRefGoogle ScholarPubMed