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Sulphydryl reagent iodoacetamide inhibits progression of meiosis and sperm transformation in mouse oocytes fertilised in vitro

Published online by Cambridge University Press:  26 September 2008

Marek Maleszewski
Marek Maleszewski
Affiliation:
Department of Embryology, Institute of Zoology, University of Warsaw, Poland.
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Summary

The effect of iodoacetamide (IA), a sulphydryl blocking agent, on fertilisation in the mouse was examined by transferring zona-free oocytes into IA (500 μM)-containing medium at various times after insemination. When inseminated oocytes were transferred into IA medium 10 min after insemination, the oocyte chromosomes remained aggregated in one or two masses and the sperm nucleus failed to decondense. When oocytes were transferred during the second meiosis, oocytes meiosis was more or less arrested. The sperm nucleus decondensed but remained blocked at an early stage of decondensation. These observations suggest that thiol groups in the oocyte's cytoplasm and perhaps microtubules of the meiotic spindle play crucial roles in the completion of meiosis and the transformation of sperm nucleus into pronucleus.

Keywords

Disulphide bondsFertilisation in vitroIodoacetamideOocyte activationSperm transformation
Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995 , pp. 75 - 79
Copyright
Copyright © Cambridge University Press 1995

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