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Staurosporine advances interblastomeric flattening of the mouse embryo

Published online by Cambridge University Press:  26 September 2008

D. Michael O'Sullivan ,
Martin H. Johnson  and
Josie M.L. McConnell
D. Michael O'Sullivan
Affiliation:
Department of Anatomy, University of Cambridge, Cambridge, UK.
Martin H. Johnson*
Affiliation:
Department of Anatomy, University of Cambridge, Cambridge, UK.
Josie M.L. McConnell
Affiliation:
Department of Anatomy, University of Cambridge, Cambridge, UK.
*
Professor M.H. Johnson, Embryo and Gamete Research Group, Department of Anatomy, Downing Street, Cambridge CB2 3DY, UK. Telephone: (0223) 333789. Fax: (0223) 333786.
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Extract

Staurosporine, an inhibitor of protein kinase activity, causes premature intercellular flattening of blastomeres but does not induce their premature polarisation. The flattening induced is calcium dependent, is reversed transiently at mitosis and requires the continuing presence of the drug. Staurosporine also blocks the decompacting effect of phorbol ester on 8-cell embryos.

Keywords

CompactionEmbryoMouseProtein kinaseStaurosporine
Type
Article
Information
Zygote , Volume 1 , Issue 2 , May 1993 , pp. 103 - 112
Copyright
Copyright © Cambridge University Press 1993

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