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Spermatozoa isolated from cat testes retain their structural integrity as well as a developmental potential after refrigeration for up to 7 days

Published online by Cambridge University Press:  03 July 2014

Sirirak Buarpung ,
Theerawat Tharasanit ,
Chommanart Thongkittidilok ,
Pierre Comizzoli  and
Mongkol Techakumphu
Sirirak Buarpung
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Theerawat Tharasanit
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Chommanart Thongkittidilok
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Pierre Comizzoli
Affiliation:
Department of Reproductive Sciences, Center for Species Survival, Smithsonian Conservation Biology Institute, Washington, District of Columbia, USA.
Mongkol Techakumphu*
Affiliation:
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
*
All correspondence to: Mongkol Techakumphu. Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. Tel: +66 2 218 9644. Fax: +66 2 2520738. e-mail: [email protected]
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Summary

The objective of this study was to compare the efficiency of preservation media for isolated feline testicular spermatozoa as well as the concentrations of bovine serum albumin (BSA) on: (1) the membrane (sperm membrane integrity (SMI)) and DNA integrity of spermatozoa; and (2) the developmental potential of spermatozoa after intracytoplasmic sperm injection (ICSI). Isolated cat spermatozoa were stored in HEPES-M199 medium (HM) or Dulbecco's phosphate-buffered saline (DPBS) at 4°C for up to 7 days. Results indicated that HM maintained a better SMI than DPBS throughout the storage periods (P > 0.05). When spermatozoa were stored in HM supplemented with BSA at different concentrations (4, 8 or 16 mg/ml), SMI obtained from HM containing 8 and 16 mg/ml BSA was higher than with 4 mg/ml BSA (P < 0.05). DNA integrity of spermatozoa stored in HM with 16 mg/ml BSA for 7 days was poorer than that of the fresh control, but the subsequent percentages of cleavage, morula, blastocyst produced by ICSI, as well as their average blastomere numbers of blastocysts, were similar (P > 0.05). In summary, cat spermatozoa immediately isolated from testicular tissue can be stored as a suspension in basic buffered medium at 4°C for up to 7 days. BSA supplementation into the medium improves membrane integrity of the spermatozoa during cold storage. Testicular spermatozoa stored in HM containing 16 mg/ml BSA retained full in vitro developmental potential after ICSI, similar to that of fresh controls even though DNA integrity had slightly declined.

Keywords

Cold storageDNA integrityICSIMembrane integrityTesticular spermatozoa
Type
Research Article
Information
Zygote , Volume 23 , Issue 5 , October 2015 , pp. 644 - 651
Copyright
Copyright © Cambridge University Press 2014 

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References

Abe, Y., Lee, D.S., Sano, H., Akiyama, K., Yanagimoto-Ueta, Y., Asano, T., Suwa, Y. & Suzuki, H. (2008). Artificial insemination with canine spermatozoa frozen in a skim milk/glucose-based extender. J. Reprod. Dev. 54, 290–4.CrossRefGoogle Scholar
Ahmadi, A. & Ng, S.C. (1999). Fertilizing ability of DNA-damaged spermatozoa. J. Exp. Zool. 284, 696–704.3.0.CO;2-E>CrossRefGoogle ScholarPubMed
Aires, V.A., Hinsch, K.D., Mueller-Schloesser, F., Bogner, K., Mueller-Schloesser, S., & Hinsch, E. (2003). In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen. Theriogenology 60, 269–79.CrossRefGoogle ScholarPubMed
Al Ahmad, M.Z., Chatagnon, G., Amirat-Briand, L., Moussa, M., Tainturier, D., Anton, M. & Fieni, F. (2008). Use of glutamine and low density lipoproteins isolated from egg yolk to improve buck semen freezing. Reprod. Domest. Anim. 43, 429–36.CrossRefGoogle ScholarPubMed
Avendano, C., Franchi, A., Duran, H. & Oehninger, S. (2010). DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome. Fertil. Steril. 94, 549–57.CrossRefGoogle ScholarPubMed
Bilodeau, J.F., Blanchette, S., Cormier, N. & Sirard, M.A. (2002). Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology 57, 1105–22.CrossRefGoogle ScholarPubMed
Blank, M., Soo, L. & Butten, J.S. (1976). Adsorption of albumin on rabbit sperm membranes. J. Membr. Biol. 29, 401–9.CrossRefGoogle ScholarPubMed
Bruemmert, J.E., Coy, R.C., Squires, E.L. & Graham, J.K. (2002). Effect of pyruvate on the function of stallion spermatozoa stored for up to 48 hours. J. Anim. Sci. 80, 12–8.CrossRefGoogle ScholarPubMed
Buarpung, S., Tharasanit, T., Comizzoli, P. & Techakumphu, M. (2012). Effects of cold storage on plasma membrane, DNA integrity and fertilizing ability of feline testicular spermatozoa. Anim. Reprod. Sci. 131, 219–27.CrossRefGoogle ScholarPubMed
Buarpung, S., Tharasanit, T., Comizzoli, P. & Techakumphu, M. (2013). Feline spermatozoa from fresh and cryopreserved testicular tissues have comparable ability to fertilize matured oocytes and sustain the embryo development after intracytoplasmic sperm injection. Theriogenology 79, 149–58.CrossRefGoogle ScholarPubMed
Colás, C., Junquera, C., Pérez-Pé, R., Cebrián-Pérez, J.A. & Muiño-Blanco, T. (2009). Ultrastructural study of the ability of seminal plasma proteins to protect ram spermatozoa against cold-shock. Microsc. Res. Tech. 72, 566–72.CrossRefGoogle ScholarPubMed
de Mercado, E., Hernandez, M., Sanz, E., Rodriguez, A., Gomez, E., Vazquez, J.M., Martinez, E.A. & Roca, J. (2009). Evaluation of L-glutamine for cryopreservation of boar spermatozoa. Anim. Reprod. Sci. 115, 149–57.CrossRefGoogle ScholarPubMed
Dondero, F., Rossi, T., Delfino, M., Imbrogno, N., Cannistra, S. & Mazzilli, F. (2006). Human semen refrigeration at +4°C: bio-kinetic characteristics. Cell Tissue Bank. 7, 61–4.CrossRefGoogle Scholar
Eckert, J. & Niemann, H. (1995). In vitro maturation, fertilization and culture to blastocysts of bovine oocytes in protein-free media. Theriogenology 43, 1211–25.CrossRefGoogle ScholarPubMed
England, G.C.W. & Ponzio, P. (1996). Comparison of the quality of frozen–thawed and cooled–rewarmed dog semen. Theriogenology 46, 165–71.CrossRefGoogle Scholar
Gañán, N., Gomendio, M. & Roldan, E.R. (2009). Effect of storage of domestic cat (Felis catus) epididymides at 5°C on sperm quality and cryopreservation. Theriogenology 72, 1268–77.CrossRefGoogle Scholar
Hereng, T.H., Elgstøen, K.B., Cederkvist, F.H., Eide, L., Jahnsen, T., Skålhegg, B.S. & Rosendal, K.R. (2011). Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa. Hum. Reprod. 26, 3249–63.CrossRefGoogle ScholarPubMed
Hermansson, U. & Axnér, E. (2007). Epididymal and ejaculated cat spermatozoa are resistant to cold shock but egg yolk promotes sperm longevity during cold storage at 4°C. Theriogenology 67, 1239–48.CrossRefGoogle Scholar
Jahnukainen, K., Ehmcke, J., Hergenrother, S.D. & Schlatt, S. (2007). Effect of cold storage and cryopreservation of immature non-human primate testicular tissue on spermatogonial stem cell potential in xenografts. Hum. Reprod. 22, 1060–7.CrossRefGoogle ScholarPubMed
Jiang, H., He, R.B., Wang, C.L. & Zhu, J. (2011). The relationship of sperm DNA fragmentation index with the outcomes of in-vitro fertilisation-embryo transfer and intracytoplasmic sperm injection. J. Obstet. Gynaecol. 31, 636–9.CrossRefGoogle ScholarPubMed
Li, C., Mizutani, E., Ono, T., Terashita, Y., Jia, X.F., Shi, H.J. & Wakayama, T. (2011). Intracytoplasmic sperm injection with mouse spermatozoa preserved without freezing for 6 months can lead to full-term development. Biol. Reprod. 85, 1183–90.CrossRefGoogle ScholarPubMed
Matsuoka, T., Imai, H., Kohno, H. & Fukui, Y. (2006). Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen–thawed ram spermatozoa. J. Reprod. Dev. 52, 675–83.CrossRefGoogle ScholarPubMed
Nang, C.F., Osman, K., Budin, S.B., Ismail, M.I., Jaffar, F.H.F., Mohamad, S.F. & Ibrahiml, S.F. (2012). Bovine serum albumin: survival and osmolarity effect in bovine spermatozoa stored above freezing point. Andrologia 44, 447–53.CrossRefGoogle ScholarPubMed
Nasr-Esfahani, M.H., Salehi, M., Razavi, S., Anjomshoa, M., Rozbahani, S., Moulavi, F. & Mardani, M. (2005). Effect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI. Reprod. Biomed. Online 11, 198–205.Google ScholarPubMed
Osman, K., Nang, C.F., Ibrahim, S.F., Budin, S.B., Jaffar, F.H.F. & Wahab, N.A.A. (2012). Albumin improved spermatozoa quality and dna integrity for freezing-free preservation. Int. J. Biol. Med. Res. 3, 1670–9.Google Scholar
Riel, J.M., Yamauchi, Y., Huang, T.T., Grove, J. & Ward, M.A. (2011). Short-term storage of human spermatozoa in electrolyte-free medium without freezing maintains sperm chromatin integrity better than cryopreservation. Biol. Reprod. 85, 536–47.CrossRefGoogle ScholarPubMed
Risopatron, J., Catalan, S., Miska, W., Schill, W.B. & Sanchez, R. (2002). Effect of albumin and polyvinyl alcohol on the vitality, motility and acrosomal integrity of canine spermatozoa incubated in vitro. Reprod. Domest. Anim. 37, 347–51.CrossRefGoogle ScholarPubMed
Russler-Long, J.A., Dickey, J.F., Richardson, M.E. & Ivey, K.W. (1991). Culture of ovine embryos in the absence of bovine serum albumin. Theriogenology 35, 383–91.CrossRefGoogle ScholarPubMed
Sananmuang, T., Techakumphu, M. & Tharasanit, T. (2010). The effects of roscovitine on cumulus cell apoptosis and the developmental competence of domestic cat oocytes. Theriogenology 73, 199–207.CrossRefGoogle ScholarPubMed
Sato, M. & Ishikawa, A. (2004). Room temperature storage of mouse epididymal spermatozoa: exploration of factors affecting sperm survival. Theriogenology 61, 1455–69.CrossRefGoogle ScholarPubMed
Siemieniuch, M. & Dubiel, A. (2007). Preservation of tom cat (Felis catus) semen in variable temperature. Anim. Reprod. Sci. 99, 135–44.CrossRefGoogle Scholar
Tateno, H., Kimura, Y. & Yanagimachi, R. (2000). Sonication per se is not as deleterious to sperm chromosomes as previously inferred. Biol. Reprod. 63, 341–6.CrossRefGoogle Scholar
Uysal, O. & Bucak, M.N. (2007). Effects of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed ram semen. Acta. Vet. Brno. 76, 383–90.CrossRefGoogle Scholar
Van Thuan, N., Wakayama, S., Kishigami, S. & Wakayama, T. (2005). New preservation method for mouse spermatozoa without freezing. Biol. Reprod. 72, 444–50.CrossRefGoogle ScholarPubMed
Varga, S., Diez, C., Fernández, L., Alvarez, J., Katchicualula, A., Hidalgo, C.O., Tamargo, C. & Carbajo, M. (2011). Culture system and long-term storage of culture media in the in vitro production of bovine embryos. Acta. Vet. Hung. 59, 129–39.CrossRefGoogle ScholarPubMed
Vick, M.M., Bateman, H.L., Lambo, C.A. & Swanson, W.F. (2012). Improved cryopreservation of domestic cat sperm in a chemically defined medium. Theriogenology 78, 2120–8.CrossRefGoogle Scholar
Wildt, D.E., Comizzoli, P., Pukazhenthi, B. & Songsasen, N. (2010). Lessons from biodiversity—the value of nontraditional species to advance reproductive science, conservation, and human health. Mol. Reprod. Dev. 77, 397–409.CrossRefGoogle ScholarPubMed
Wood, T.C., Byers, A.P., Jennette, B.E. & Wildt, D.E. (1995). Influence of protein and hormone supplementation on in vitro maturation and fertilization of domestic cat eggs. J. Reprod. Fertil. 104, 315–23.CrossRefGoogle ScholarPubMed
Yang, Y. & Honaramooz, A. (2010). Effects of medium and hypothermic temperatures on preservation of isolated porcine testis cells. Reprod. Fertil. Dev. 22, 523–32.CrossRefGoogle ScholarPubMed
Yang, Y., Steeg, J. & Honaramooz, A. (2010). The effects of tissue sample size and media on short-term hypothermic preservation of porcine testis tissue. Cell. Tissue. Res. 340, 397–406.CrossRefGoogle ScholarPubMed
Zambelli, D. & Cunto, M. (2006). Semen collection in cats: techniques and analysis. Theriogenology 66, 159–65.CrossRefGoogle ScholarPubMed
Zavos, P.M., Goodpasture, J.C., Zaneveld, L.J. & Cohen, M.R. (1980). Motility and enzyme activity of human spermatozoa stored for 24 hours at + 5°C and –196°C. Fertil. Steril. 34, 607–9.CrossRefGoogle Scholar
Zeng, W., Snedaker, A.K., Megee, S., Rathi, R., Chen, F., Honaramooz, A. & Dobrinski, I. (2009). Preservation and transplantation of porcine testis tissue. Reprod. Fertil. Dev. 21, 489–97.CrossRefGoogle ScholarPubMed