Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-18T18:13:36.037Z Has data issue: false hasContentIssue false
  • English
  • Français

Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm

Published online by Cambridge University Press:  16 July 2018

Blanca-Estela Galindo ,
Takuya Nishigaki ,
Esmeralda Rodríguez ,
Daniel Sánchez ,
Camen Beltrán  and
Alberto Darszon
Blanca-Estela Galindo
Affiliation:
Department of Molecular Genetics and Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Apdo. 510-3, Cuernavaca, Mor. 62250, Mexico
Takuya Nishigaki
Affiliation:
Department of Molecular Genetics and Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Apdo. 510-3, Cuernavaca, Mor. 62250, Mexico
Esmeralda Rodríguez
Affiliation:
Department of Molecular Genetics and Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Apdo. 510-3, Cuernavaca, Mor. 62250, Mexico
Daniel Sánchez
Affiliation:
Department of Molecular Genetics and Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Apdo. 510-3, Cuernavaca, Mor. 62250, Mexico
Camen Beltrán
Affiliation:
Department of Molecular Genetics and Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Apdo. 510-3, Cuernavaca, Mor. 62250, Mexico
Alberto Darszon
Affiliation:
Department of Molecular Genetics and Physiology, Institute of Biotechnology, National Autonomous University of Mexico, Apdo. 510-3, Cuernavaca, Mor. 62250, Mexico
Get access Rights & Permissions [Opens in a new window]

Extract

We are studying the regulation of ion transport in sperm physiology. Sperm ion permeability is modulated by components from the outer layer of the egg which, depending on the species, regulate sperm motility, Chemotaxis and the acrosome reaction (AR). This reaction is required for sperm to fertilise the egg in many species from sea urchins to man (Darszon et al., 1999).

Speract, a decapeptide from the external layer of Strongylocentrotus purpuratus sea urchin eggs, influences sperm respiration, motility and possibly the AR. Signal transduction starts when speract binds to a protein of 77 kDa closely coupled to sperm guanylyl cyclase (Garbers, 1989). Our recent receptor binding experiments using fluorescent-labelled speract (fluorescein and rhodamine) have allowed estimates of the association (kon 2.4 × 107 M−1s−1) and dissociation rate constants (koff 1.3 × 10−4 s−1). Furthermore, studies with fluorescent speract analogues indicate that the receptor undergoes conformational changes that depend on intracellular pH (pHi). The overall results are consistent with the possibility that speract may induce in sea urchin sperm a hyperactivated-like flagellar movement inside the jelly coat to accelerate sperm penetration through this layer.

Type
Special Lecture for Citizens
Information
Zygote , Volume 8 , supplement S1: Proceedings of the International Symposium on Fertilization and Development of Sea Urchin and Marine Invertebrates , December 1999 , pp. S20 - S21
Copyright
Copyright © Cambridge University Press 1999

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Babcock, D.F., Bosma, M.M., Battaglia, D.E. & Darszon, A. (1992). Proc. Natl. Acad. Sci. USA 89, 6001–5.CrossRefGoogle Scholar
Beltrán, C., Zapata, O. & Darszon, A. (1996). Biochemistry 35, 7591–8.CrossRefGoogle Scholar
Darszon, A., Labarca, P., Nishigaki, T. & Espinosa, F. (1999). Physiol. Rev. 79, 481–510.CrossRefGoogle Scholar
Galindo, B.E., Beltrán, C., Cragoe, E.J. Jr & Darszon, A. (2000). Dev. Biol., 221, 285–94.CrossRefGoogle Scholar
Garbers, D.L. (1989). Annu. Rev. Biochem. 58, 719–42.CrossRefGoogle Scholar
Koch, K.W. & Kaupp, U.B. (1985). J. Biol. Chem. 260, 6788–800.CrossRefGoogle Scholar
Lee, H.C. (1984). J. Biol. Chem. 259, 4957–63.CrossRefGoogle Scholar
Lee, H.C. & Garbers, D.L. (1986). J. Biol. Chem. 261, 16026–32.CrossRefGoogle Scholar
Negulescu, P.A., Harooturian, A., Tsien, R. & Manchen, T.B. (1990). Cell Reg. 1, 259–68.CrossRefGoogle Scholar
Schackmann, R.W. (1989). In The Cell Biology of Fertilization, ed. Schatten, H. & Schatten, G., pp. 3–28. San Diego: Academic Press.CrossRefGoogle Scholar