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SOCE proteins, STIM1 and Orai1, are localized to the cleavage furrow during cytokinesis of the first and second cell division cycles in zebrafish embryos

Published online by Cambridge University Press:  05 October 2016

Ching Man Chan ,
Jacqueline T. M. Aw ,
Sarah E. Webb  and
Andrew L. Miller
Ching Man Chan
Affiliation:
Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, People's Republic of China.
Jacqueline T. M. Aw
Affiliation:
Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, People's Republic of China.
Sarah E. Webb
Affiliation:
Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, People's Republic of China.
Andrew L. Miller*
Affiliation:
Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, People's Republic of China. Marine Biological Laboratory, Woods Hole, MA 02543, USA.
*
All correspondence to: Andrew L. Miller. Division of Life Science & State Key Laboratory of Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, People's Republic of China. E-mail: [email protected]
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Summary

In zebrafish embryos, distinct Ca2+ transients are localized to the early cleavage furrows during the first few cell division cycles. These transients are generated mainly by release via IP3Rs in the endoplasmic reticulum, and they are necessary for furrow positioning, propagation, deepening and apposition. We previously showed, via the use of inhibitors, that store-operated Ca2+ entry (SOCE) also appears to be essential for maintaining the IP3R-mediated elevated levels of [Ca2+]i for the extended periods required for the completion of successful furrow deepening and daughter cell apposition in these large embryonic cells. Here, newly fertilized, dechorionated embryos were fixed at various times during the first and second cell division cycles and immunolabelled with antibodies to STIM1 and/or Orai1 (key components of SOCE). We show that both of these proteins have a dynamic pattern of localization during cytokinesis of the first two cell division cycles. These new data help to support our previous inhibitor results, and provide additional evidence that SOCE contributes to the maintenance of the sustained elevated Ca2+ that is required for the successful completion of cytokinesis in the large cells of embryonic zebrafish.

Keywords

Ca2+ signallingCytokinesisSTIM1 and Orai1SOCEZebrafish embryos
Type
Research Article
Information
Zygote , Volume 24 , Issue 6 , December 2016 , pp. 880 - 889
Copyright
Copyright © Cambridge University Press 2016 

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