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Sebox plays an important role during the early mouse oogenesis in vitro

Published online by Cambridge University Press:  18 July 2012

Dafne Linda Moreno ,
Zayil Salazar ,
Miguel Betancourt ,
Eduardo Casas ,
Yvonne Ducolomb ,
Cristina González  and
Edmundo Bonilla
Dafne Linda Moreno
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Zayil Salazar
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Miguel Betancourt
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Eduardo Casas
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Yvonne Ducolomb
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Cristina González
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Edmundo Bonilla*
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, Mexico.
*
All correspondence to: Edmundo Bonilla. Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, Mexico. Tel.: +52 55 58046557. Fax: +52 55 58044727. e-mail: mundo@xanum.uam.mx
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Summary

Oogenesis is a highly complex process that requires the exquisite temporal and spatial regulation of gene expression at multiple levels. Skin–embryo–brain–oocyte homeobox (Sebox) gene encodes a transcription factor that is highly expressed in germinal vesicle stage oocytes and that plays an essential role in early embryogenesis at the 2-cell stage in the mouse. As Sebox is also expressed in mouse fetal ovaries, the aim of the present study was to study its role during the early oogenesis in vitro. Expression of Sebox was low in 15.5 to 17.5 days post coitum (dpc) ovaries, showed a peak at 18.5 dpc and then its expression decreased dramatically in newborn ovaries. Sebox expression was efficiently knocked down (>80%) in fetal mouse ovary explants in culture using RNAi technology. When fetal ovary explants were transfected with Sebox-specific RNAi, the number of oocytes at germinal vesicle stage and showing a diameter of 40–70 μm was decreased significantly to 75% after 7 days of culture relative to the negative control, and to 22.4% after 10 days of culture, thus indicating that Sebox plays an important role in the early oogenesis in mice.

Keywords

Oocyte developmentOogenesisRNAiSebox
Type
Research Article
Information
Zygote , Volume 22 , Issue 1 , February 2014 , pp. 64 - 68
Copyright
Copyright © Cambridge University Press 2012 

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