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Sebox plays an important role during the early mouse oogenesis in vitro

Published online by Cambridge University Press:  18 July 2012

Dafne Linda Moreno
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Zayil Salazar
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Miguel Betancourt
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Eduardo Casas
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Yvonne Ducolomb
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Cristina González
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Edmundo Bonilla*
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, Mexico.
*
All correspondence to: Edmundo Bonilla. Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, Mexico. Tel.: +52 55 58046557. Fax: +52 55 58044727. e-mail: [email protected]

Summary

Oogenesis is a highly complex process that requires the exquisite temporal and spatial regulation of gene expression at multiple levels. Skin–embryo–brain–oocyte homeobox (Sebox) gene encodes a transcription factor that is highly expressed in germinal vesicle stage oocytes and that plays an essential role in early embryogenesis at the 2-cell stage in the mouse. As Sebox is also expressed in mouse fetal ovaries, the aim of the present study was to study its role during the early oogenesis in vitro. Expression of Sebox was low in 15.5 to 17.5 days post coitum (dpc) ovaries, showed a peak at 18.5 dpc and then its expression decreased dramatically in newborn ovaries. Sebox expression was efficiently knocked down (>80%) in fetal mouse ovary explants in culture using RNAi technology. When fetal ovary explants were transfected with Sebox-specific RNAi, the number of oocytes at germinal vesicle stage and showing a diameter of 40–70 μm was decreased significantly to 75% after 7 days of culture relative to the negative control, and to 22.4% after 10 days of culture, thus indicating that Sebox plays an important role in the early oogenesis in mice.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2012 

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References

Ballow, D.J., Xin, Y., Choi, Y., Pangas, S.A. & Rajkovic, A. (2006). Sohlh2 is a germ cell-specific bHLH transcription factor. Gene Expr. Patterns 6, 1014–8.CrossRefGoogle ScholarPubMed
Bonilla, E. & del Mazo, J. (2010). Deregulation of the Sod1 and Nd1 genes in mouse fetal oocytes exposed to mono-(2-ethylhexyl)phthalate (MEHP). Reprod. Toxicol. 30, 387–92.Google Scholar
Bonilla, E. & Xu, E.Y. (2008). Identification and characterization of novel mammalian spermatogenic genes conserved from fly to human. Mol. Hum. Reprod. 14, 137–42.Google Scholar
Choi, Y., Qin, Y., Berger, M.F., Ballow, D.J., Bulyk, M.L. & Rajkovic, A. (2007). Microarray analyses of newborn mouse ovaries lacking Nobox. Biol. Reprod. 77, 312–9.Google Scholar
Choi, Y. & Rajkovic, A. (2006). Characterization of NOBOX DNA binding specificity and its regulation of Gdf9 and Pou5f1 promoters. J. Biol. Chem. 281, 35747–56.Google Scholar
Choi, Y., Yuan, D. & Rajkovic, A. (2008). Germ cell-specific transcriptional regular Sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression. Biol. Reprod. 79, 1176–82.CrossRefGoogle Scholar
Cinquanta, M., Rovescalli, A.C., Kozak, C.A. & Nirenberg, M. (2000). Mouse Sebox homeobox gene expression in skin, brain, oocytes, and two-cell embryos. Proc. Natl. Acad. Sci. USA 97, 8904–9.Google Scholar
De Felici, M. (1998). In vitro culture systems form germ cells from mouse embryo: primordial germ cells and oocytes. In Reproductive Toxicology: In Vitro Germ Cell Developmental Toxicology from Science to Social and Industrial Demand. (ed. Jesús del Mazo), pp. 41–7. New York: Plenum Press.CrossRefGoogle Scholar
Hong, S., Choi, I., Woo, J.M., Oh, J., Kim, T., Choi, E., Kim, T.W., Jung, Y.K., Kim, D.H., Sun, C.H., Yi, G.S., Eddy, E.M. & Cho, C. (2005). Identification and integrative analysis of 28 novel genes specifically expressed and developmentally regulated in murine spermatogenic cells. J. Biol. Chem. 280, 7685–93.CrossRefGoogle ScholarPubMed
Jagarlamudi, K. & Rajkovic, A. (2012). Oogenesis: transcriptional regulators and mouse models. Mol. Cell. Endocrinol. 356, 31–9.CrossRefGoogle ScholarPubMed
Joshi, S., Davies, H., Sims, L.P., Levy, S.E. & Dean, J. (2007). Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor. BMC Dev. Biol. 7, 67.Google Scholar
Kim, K.H., Kim, E.Y. & Lee, K.A. (2008). SEBOX is essential for early embryogenesis at the two-cell stage in the mouse. Biol. Reprod. 1192–201.Google Scholar
Pepling, M.E. & Spradling, A.C. (2001). Mouse ovarian germ cell cysts undergo programmed breakdown to form primordial follicles. Dev. Biol. 234, 339–51.Google Scholar
Rajkovic, A., Pangas, S.A., Ballow, D., Suzumori, N. & Matzuk, M.M. (2004). NOBOX deficiency disrupts early folliculogenesis and oocyte-specific gene expression. Science 305, 1157–9.Google Scholar
Rozen, S. & Skaletsky, H. (2000). Primer3 on the WWW for general users and for biologist programmers. Meth Mol Biol, 132, 365–8.Google Scholar
Soyal, S.M., Amleh, A. & Dean, J. (2000). FIGalpha, a germ cell-specific transcription factor required for ovarian follicle formation. Development 127, 4645–54.Google Scholar
Tong, Z.-B., Gold, L., Pfeifer, K.E., Dorward, H., Lee, E., Bondy, C.A., Dean, J. & Nelson, L.M. (2000). Mater, a maternal effect gene required for early embryonic development in mice. Nat. Genet. 26, 267–8.Google Scholar
Wu, X., Viveiros, M.M., Eppig, J.J., Bai, Y., L., F.S. & Matzuk, M.M. (2003). Zygote arrest 1 (Zar1) is a novel maternal-effect gene critical for the oocyte-to-embryo transition. Nat. Genet. 33, 187–91.Google Scholar
Xu, B., Hua, J., Zhang, Y., Jiang, X., Zhang, H., Ma, T., Zheng, W., Sun, R., Shen, W., Sha, J., Cooke, H.J. & Shi, Q. (2011). Proliferating cell nuclear antigen (PCNA) regulates primordial follicle assembly by promoting apoptosis of oocytes in fetal and neonatal mouse ovaries. PLoS One 6, e16046.CrossRefGoogle ScholarPubMed
Yan, C., Wang, P., DeMayo, J., DeMayo, F.J., Elvin, J.A., Carino, C., Prasad, S.V., Skinner, S.S., Dunbar, B.S., Dube, J.L., Celeste, A.J. & Matzuk, M.M. (2001). Synergistic roles of bone morphogenetic protein 15 and growth differentiation factor 9 in ovarian function. Mol. Endocrinol. 15, 854–66.CrossRefGoogle ScholarPubMed