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RNase A activity in Japanese quail oocytes

Published online by Cambridge University Press:  26 September 2008

Urszula Stepińska ,
Arleta Malewska  and
Bożenna Olszańska
Urszula Stepińska*
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiecn. Warsaw, 05-551 Mroków, Poland.
Arleta Malewska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiecn. Warsaw, 05-551 Mroków, Poland.
Bożenna Olszańska
Affiliation:
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiecn. Warsaw, 05-551 Mroków, Poland.
*
U. Stepińka, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-551 Mroków, Poland. Telephone: +48 (22) 7561 711. Fax: +48 (22) 7561 699.
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Summary

The presence of RNase A activity was studied in vitro in homogenates of quail oocytes and early embryos using [3H]poly(U) as a substrate. The activity was measured by adsorption of the undegraded substrate onto DE-81 filter paper discs and by chromatographic separation on a Sephadex G-50 column. RNase A activity examined by these methods was almost undetectable in quail previtellogenic, vitellogenic and ovulated oocytes as well as in the embryos from laid eggs. It is estimated to be about 1.1 × 10−5 Kunitz units per ovulated oocyte. Higher activity starts to appear in gastrulating embryos. These findings are discussed in relation to other studies demonstrating the high stability of maternal RNA during early development, especially in growing oocytes.

Keywords

Maternal RNAOocyteQuailRNA degradationRNase A activity
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 219 - 227
Copyright
Copyright © Cambridge University Press 1996

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