Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-24T02:32:02.821Z Has data issue: false hasContentIssue false
  • English
  • Français

Reproductive strategies and chromosomal aberrations affect survival in the Rivuliid fish Hypsolebias sertanejo

Published online by Cambridge University Press:  05 October 2020

Mariana Machado Evangelista ,
Elizabeth Romagosa ,
Diógenes Henrique Siqueira-Silva Open the ORCID record for Diógenes Henrique Siqueira-Silva [Opens in a new window] ,
George Shigueki Yasui ,
Takafumi Fujimoto Open the ORCID record for Takafumi Fujimoto [Opens in a new window]  and
José Augusto Senhorini Open the ORCID record for José Augusto Senhorini [Opens in a new window]
Mariana Machado Evangelista
Affiliation:
Aquaculture Center, São Paulo State University, Jaboticabal, São Paulo, Brazil
Elizabeth Romagosa
Affiliation:
Fishery Institute, SAA, SP, Água Branca, São Paulo, SP, Brasil
Diógenes Henrique Siqueira-Silva*
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LANEC), Biology Faculty (FACBIO), University of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
George Shigueki Yasui
Affiliation:
National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
Takafumi Fujimoto
Affiliation:
Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan
José Augusto Senhorini
Affiliation:
National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Pirassununga, São Paulo, Brazil
*
Author for correspondence: Diógenes Henrique Siqueira-Silva. Group of Studies on the Reproduction of Amazon fish (GERPA/LANEC), Biology Faculty (FACBIO), University of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Rivulidae comprises a family of fish largely distributed in Brazil that includes 201 species, of which 125 are considered endangered. This fact emphasizes the need for development of conservation strategies including studies on genetics and reproduction. In this paper, we describe aspects of biology and reproduction of the rivuliid species Hypsolebias sertanejo. We outline the reproductive behaviour of this species under laboratory conditions, analyze ploidy status by flow cytometry, describe reproductive behaviour and performance and test dry and wet incubation of eggs. Although H. sertanejo showed well known patterns of reproductive behaviour, we verified many peculiarities inherent to its reproductive biology. As expected, most individuals were diploid (87.71%), however 14.29% were considered mosaics. Although no sterility was observed within mosaics, infertility of these fish was not fully evaluated. Hatching rate of the eggs collected was very low following both dry and wet incubation (5.04 and 3.79%, respectively). These results provide interesting information regarding the reproductive success of this species, and suggest that chromosomal abnormalities described may reduce the survival of H. sertanejo under natural conditions, limiting the perpetuation of this species, and emphasizing the need for more preservation efforts, including artificial propagation and gene banking.

Keywords

BiotechnologyFish biologyFish preservationMosaicismReproduction
Type
Research Article
Information
Zygote , Volume 29 , Issue 1 , February 2021 , pp. 20 - 26
Copyright
© The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abilhoa, V, Vitule, JRS and Bornatowski, H (2010). Feeding ecology of Rivulus luelingi (Aplocheiloidei: Rivulidae) in a coastal Atlantic rainforest stream, southern Brazil. Neotrop Ichthyol 8, 813–8.CrossRefGoogle Scholar
Adamov, NSM, Nascimento, NF, Maciel, ECS, Pereira-Santos, M, Senhorini, JA, Calado, LL, Evangelista, MM, Nakaghi, LSO, Guerrero, AHM, Fujimoto, T and Yasui, GS (2017). Triploid induction in the yellowtail tetra, Astyanax altiparanae, using temperature shock: tools for conservation and aquaculture. J World Aquacult Soc 48, 741–50.CrossRefGoogle Scholar
Aegerter, S and Jalabert, B (2004). Effects of post-ovulatory oocyte ageing and temperature on egg quality and on the occurrence of triploid fry in rainbow trout, Oncorhynchus mykiss Aquaculture 231, 59–71.CrossRefGoogle Scholar
Avise, JC and Tatarenkov, A (2015). Population genetics and evolution of the mangrove rivulus Kryptolebias marmoratus, the world’s only self-fertilizing hermaphroditic vertebrate, J Fish Biol 87, 519–38.CrossRefGoogle ScholarPubMed
Belote, DF and Costa, WJEM (2002). Reproductive behavior patterns in the neotropical annual fish genus Simpsonichthys Carvalho, 1959 (Cyprinodontiformes, Rivulidae): description and phylogenetic implications: Oficina Grafica da Universidade do Brasil.Google Scholar
Cassel, M, Mehanna, M, Mateus, L, and Ferreira, A (2013). Gametogenesis and reproductive cycle of Melanorivulusaff punctatus (Boulenger, 1895) (Cyprinodontiformes, Rivulidae) in Chapada dos Guimarães, Mato Grosso, Brazil. Neotrop Ichthyol 11, 241–4.CrossRefGoogle Scholar
Costa, WJ (2002). The neotropical seasonal fish genus Nematolebias (Cyprinodontiformes: Rivulidae: Cynolebiatinae): taxonomic revision with description of a new species. Ichthyol Explor Fresh 13, 41–52.Google Scholar
Costa, WJ (2003). Family Rivulidae (South American annual fishes). In Check List of the Freshwater Fishes of South and Central America (eds Reis, Roberto E., Kullander, Sven O., and Ferraris, Carl J. Jr), p. 729. Edipucrs, Porto Alegre, Brazil.Google Scholar
Costa, WJ and Amorim, PF (2014). Integrative taxonomy and conservation of seasonal killifishes, Xenurolebias (Teleostei: Rivulidae), and the Brazilian Atlantic Forest. Syst Biodivers 12, 350–65.CrossRefGoogle Scholar
Costa, WJ, Amorim, PF and Mattos, JL (2012). Species delimitation in annual killifishes from the Brazilian Caatinga, the Hypsolebias flavicaudatus complex (Cyprinodontiformes: Rivulidae): implications for taxonomy and conservation. Syst Biodivers 10, 71–91.CrossRefGoogle Scholar
Costa, WJEM, Amorim, PF and Mattos, JLO (2016). Molecular phylogeny and evolution of internal fertilization in South American seasonal cynopoeciline killifishes. Mol Phylogenet Evol 95, 94–9.CrossRefGoogle ScholarPubMed
Errea, A and Danulat, E (2001). Growth of the annual fish, Cynolebias viarius (Cyprinodontiformes), in the natural habitat compared to laboratory conditions. Environ Biol Fishes 61, 261–8.CrossRefGoogle Scholar
Flajšhans, M, Kohlmann, K and Rab, P (2007). Autotriploid tench Tinca tinca (L.) larvae obtained by fertilization of eggs previously subjected to postovulatory ageing in vitro and in vivo . J Fish Biol 71, 868–76.CrossRefGoogle Scholar
Fricke, R, Eschmeyer, WN and Van der Laan, R (2018). Catalog of fishes: genera, species, references. California Academy of Sciences, San Francisco, CA, USA. http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp.Google Scholar
Fujimoto, T, Yasui, GS, Yoshikawa, H, Yamaha, E and Arai, K (2008). Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization of Misgurnus anguillicaudatus female with M. mizolepis male. J Appl Ichthyol 24, 430–7.CrossRefGoogle Scholar
García, D, Loureiro, M and Tassino, N (2008). Reproductive behavior in the annual fish Austrolebias reicherti Loureiro & García 2004 (Cyprinodontiformes: Rivulidae). Neotrop Ichthyol 6, 243–8.CrossRefGoogle Scholar
Garcia-Abiado, MAR, Lynch, WE Jr, Dabrowski, K and Hartman, T (2001). Use of thermal and pressure shocks to induce triploid hybrid saugeyes. N Am J Aquacult 63, 243–8.2.0.CO;2>CrossRefGoogle Scholar
Hamasaki, M, Takeuchi, Y, Miyaki, K and Yoshizaki, G (2013). Gonadal development and fertility of triploid grass puffer Takifugu niphobles induced by cold shock treatment. Mar Biotechnol 15, 133–44.CrossRefGoogle ScholarPubMed
ICMBio (2018). Livro Vermelho da Fauna Brasileira Ameaçada de Extinção: Vol. I / 1. edn. Brasília, MMA, 492 pp.Google Scholar
Jara, F, Soto, D and Palma, R (1995). Reproduction in captivity of the endangered killifish Orestias ascotanensis (Teleostei: Cyprinodontidae). Copeia 1995, 226–8.CrossRefGoogle Scholar
Kalbassi, MR, Dorafshan, S, Pourkazemi, M and Amiri, BM (2009). Triploidy induction in the Caspian salmon, Salmo trutta caspius, by heat shock. J Appl Ichthyol 25, 104–7.CrossRefGoogle Scholar
Keppeler, FW, Lanés, LEK, Rolon, AS, Stenert, C and Maltchik, L (2013). The diet of Cynopoecilus fulgens Costa, 2002 (Cyprinodontiformes: Rivulidae) in Southern Brazil wetlands. Ital J Zool 80, 291–302.CrossRefGoogle Scholar
Kim, BM, Lee, BY, Lee, JH, Rhee, JS and Lee, JS (2016). Conservation of Hox gene clusters in the self-fertilizing fish Kryptolebias marmoratus (Cyprinodontiformes; Rivulidae). J Fish Biol 88, 1249–56.CrossRefGoogle Scholar
Linhart, O, Rodina, M, Flajšhans, M, Mavrodiev, N, Nebesarova, J, Gela, D, and Kocour, M (2006). Studies on sperm of diploid and triploid tench, Tinca tinca (L.). Aquac Int 14, 9–25.CrossRefGoogle Scholar
Markofsky, J and Matias, JR (1977). The effects of temperature and season of collection on the onset and duration of diapause in embryos of the annual fish Nothobranchius guentheri . J Exp Zool 202, 49–56.CrossRefGoogle ScholarPubMed
Nascimento, WSD, Bezerra, JG, Lima-Filho, PA, Yamamoto, ME, Chellapa, S and Molina, WF (2014). Karyotype patterns of Hypsolebias antenori (Cyprinodontiformes: Rivulidae): an endangered killifish of the semiarid region of Brazil. Sci World J 2014, 862434.CrossRefGoogle ScholarPubMed
Passos, C, Tassino, B, Reyes, F and Rosenthal, GG (2014). Seasonal variation in female mate choice and operational sex ratio in wild populations of an annual fish, Austrolebias reicherti . PLoS One 9, e101649.CrossRefGoogle ScholarPubMed
Rosa, RS and Lima, FCTL (2008). Os peixes brasileiros ameaçados de extinção, In Livro Vermelho Da Fauna Brasileira Ameaçada de Extinção, p. 1420.Google Scholar
Samarin, AM, Policar, T and Lahnsteiner, F (2015). Fish oocyte ageing and its effect on egg quality. Rev Fish Sci Aquac 23, 302–14.CrossRefGoogle Scholar
Samarin, AM, Blecha, M, Uzhytchak, M, Bytyutskyy, D, Zarski, D, Flajšhans, M and Policar, T (2016). Post-ovulatory and post-stripping oocyte ageing in northern pike, Esox lucius (Linnaeus, 1758), and its effect on egg viability rates and the occurrence of larval malformations and ploidy anomalies. Aquaculture 450, 431–8.CrossRefGoogle Scholar
Schalk, CM, Montaña, CG and Lisbon, ME (2014). Reproductive strategies of two neotropical killifish, Austrolebias vandenbergi and Neofundulus ornatipinnis (Cyprinodontiformes: Rivulidae) in the Bolivian Gran Chaco. Rev Biol Trop 62, 102–11.CrossRefGoogle ScholarPubMed
Shibatta, OA (2006). Comportamento social do pirá-brasília, Simpsonichthys boitonei Carvalho (Cyprinodontiformes, Rivulidae). Rev Bras Zool 2 23, 375–80.CrossRefGoogle Scholar
Volcan, MV, Lanés, LEK and Gonçalves, AC (2010). Threatened fishes of the world: Austrolebias univentripinnis Costa and Cheffe 2005 (Cyprinodontiformes: Rivulidae). Environ Biol Fishes 87, 319–20.CrossRefGoogle Scholar
Volcan, MV, Gonçalves, AC and Lanés, LEK (2011). Distribution, habitat and conservation status of two threatened annual fishes (Rivulidae) from southern Brazil. Endanger Species Res 13, 79–85.CrossRefGoogle Scholar
Wourms, JP (2011). The developmental biology of annual fishes. III. Pre-embryonic and embryonic diapause of variable duration in the eggs of annual fishes. Endanger Species Res 13, 79–85.Google Scholar
Xavier, PL, Senhorini, JA, Pereira-Santos, M, Fujimoto, T, Shimoda, E, Silva, LA, Dos Santos, AS and Yasui, GS (2017). A flow cytometry protocol to estimate DNA content in the yellowtail tetra Astyanax altiparanae . Front Genet 8, 131.CrossRefGoogle ScholarPubMed
Yasui, GS, Arias-Rodriguez, L, Fujimoto, T and Arai, K (2009). A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species. Anim Reprod Sci 116, 335–45.CrossRefGoogle ScholarPubMed