Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-24T05:07:50.241Z Has data issue: false hasContentIssue false
  • English
  • Français

Regulation of embryonic size in early mouse development in vitro culture system

Published online by Cambridge University Press:  18 January 2013

Tomoka Hisaki ,
Ikuma Kawai ,
Koji Sugiura ,
Kunihiko Naito  and
Kiyoshi Kano
Tomoka Hisaki
Affiliation:
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, University of Tokyo, Tokyo 113–8657, Japan.
Ikuma Kawai
Affiliation:
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, University of Tokyo, Tokyo 113–8657, Japan.
Koji Sugiura
Affiliation:
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, University of Tokyo, Tokyo 113–8657, Japan.
Kunihiko Naito
Affiliation:
Laboratory of Applied Genetics, Graduate School of Agricultural and Life Science, University of Tokyo, Tokyo 113–8657, Japan.
Kiyoshi Kano*
Affiliation:
Laboratory of Developmental Biology, Joint-Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan. Biomedical Science Center for Translational Research (BSCTR), United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan.
*
All correspondence to: Kiyoshi Kano. Laboratory of Developmental Biology, Joint-Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi 753–8515, Japan. e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Mammals self-regulate their body size throughout development. In the uterus, embryos are properly regulated to be a specific size at birth. Previously, size and cell number in aggregated embryos, which were made from two or more morulae, and half embryos, which were halved at the 2-cell stage, have been analysed in vivo in preimplantation and post-implantation development in mice. Here, we examined whether or not the mouse embryo has the capacity to self-regulate growth using an in vitro culture system. To elucidate embryonic histology, cells were counted in aggregated or half embryos in comparison with control embryos. Both double- and triple-aggregated embryos contained more cells than did control embryos during all culture periods, and the relative growth ratios showed no growth inhibition in an in vitro culture system. Meanwhile, half embryos contained fewer cells than control embryos, but the number grew throughout the culture period. Our data suggest that the growth of aggregated embryos is not affected and continues in an in vitro culture system. On the other hand, the growth of half embryos accelerates and continues in an in vitro culture system. This situation, in turn, implied that post-implantation mouse embryos might have some potential to regulate their own growth and size as seen by using an in vitro culture system without uterus factors. In conclusion, our results indicated that embryos have some ways in which to regulate their own size in mouse early development.

Keywords

Aggregated embryoCell numberHalf embryoMouseSize regulation
Type
Research Article
Information
Zygote , Volume 22 , Issue 3 , August 2014 , pp. 340 - 347
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allen, W.R. & Pashen, R.L. (1984). Production of monozygotic (identical) horse twins by embryo micromanipulation. J. Reprod. Fertil. 71, 607–13.CrossRefGoogle ScholarPubMed
Beck, F., Erler, T., Russell, A. & James, R. (1995). Expression of Cdx-2 in the mouse embryo and placenta: possible role in patterning of the extra-embryonic membranes. Dev. Dyn. 204, 219–27.CrossRefGoogle ScholarPubMed
Buehr, M. & McLaren, A. (1974). Size regulation in chimaeric mouse embryos. J. Embryol. Exp. Morphol. 31, 229–34.Google ScholarPubMed
Hogan, B., Beddington, R., Costantini, F. & Lacy, E. (1994). Manipulating the Mouse Embryo. Cold Spring Harbor Laboratory Press: New York.Google Scholar
Hoppe, P.C. & Whitten, W.K. (1972). Does X chromosome inactivation occur during mitosis of first cleavage? Nature 239, 520.CrossRefGoogle ScholarPubMed
Lewis, N.E. & Rossant, J. (1982). Mechanism of size regulation in mouse embryo aggregates. J. Embryol. Exp. Morphol. 72, 169–81.Google ScholarPubMed
Markert, C.L. & Petters, R.M. (1978). Manufactured hexaparental mice show that adults are derived from three embyronic cells. Science 202, 56–8.CrossRefGoogle ScholarPubMed
Mintz, B. (1964). Formation of genetically mosaic mouse embryos, and early development of ‘lethal (T12/T12)-normal’ mosaics. J. Exp. Zool. 157, 273–92.CrossRefGoogle ScholarPubMed
Mintz, B. (1965). Genetic mosaicism in adult mice of quadriparental lineage. Science 148, 1232–3.CrossRefGoogle ScholarPubMed
Moore, N.W., Adams, C.E. & Rowson, L.E. (1968). Developmental potential of single blastomeres of the rabbit egg. J. Reprod. Fertil. 17, 527–31.CrossRefGoogle ScholarPubMed
Nicholas, J.S. & Hall, B.V. (1942). Experiments on developing rats. II. The development of isolated blastomeres and fused eggs. J. Exp. Zool. 90, 441–59.CrossRefGoogle Scholar
Nicolson, G.L., Yanagimachi, R. & Yanagimachi, H. (1975). Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs. J. Cell. Biol. 66, 263–74.CrossRefGoogle ScholarPubMed
Pesce, M. & Scholer, H.R. (2001). Oct-4: gatekeeper in the beginnings of mammalian development. Stem Cells 19, 271–8.CrossRefGoogle ScholarPubMed
Petters, R.M. & Markert, C.L. (1980). Production and reproductive performance of hexaparental and octaparental mice. J. Heredity 71, 70–4.CrossRefGoogle ScholarPubMed
Petters, R.M. & Mettus, R.V. (1984). Survival rate to term of chimeric morulae produced by aggregation of five to nine embryos in the mouse, Mus musculus . Theriogenology 22, 167–74.CrossRefGoogle ScholarPubMed
Power, M.A. & Tam, P.P. (1993). Onset of gastrulation, morphogenesis and somitogenesis in mouse embryos displaying compensatory growth. Anat. Embryol. (Berl). 187, 493–504.CrossRefGoogle ScholarPubMed
Rands, G.F. (1985). Cell allocation in half- and quadruple-sized preimplantation mouse embryos. J. Exp. Zool. 236, 67–70.CrossRefGoogle ScholarPubMed
Rands, G.F. (1986a). Size regulation in the mouse embryo. I. The development of quadruple aggregates. J. Embryol. Exp. Morphol. 94, 139–48.Google ScholarPubMed
Rands, G.F. (1986b). Size regulation in the mouse embryo. II. The development of half embryos. J. Embryol. Exp. Morphol. 98, 209–17.Google ScholarPubMed
Snow, M.H. (1981). Growth and its control in early mammalian development. Br. Med. Bull. 37, 221–6.CrossRefGoogle ScholarPubMed
Tarkowski, A.K. (1959). Experiments on the development of isolated blastomeres of mouse eggs. Nature 184, 1286–7.CrossRefGoogle Scholar
Tarkowski, A.K. (1961). Mouse chimaeras developed from fused eggs. Nature 190, 857–60.CrossRefGoogle ScholarPubMed
Tarkowski, A.K. & Wroblewska, J. (1967). Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage. J. Embryol. Exp. Morphol. 18, 155–80.Google Scholar
Tsunoda, Y. & McLaren, A. (1983). Effect of various procedures on the viability of mouse embryos containing half the normal number of blastomeres. J. Reprod. Fertil. 69, 315–22.CrossRefGoogle ScholarPubMed
Willadsen, S.M. (1980). The viability of early cleavage stages containing half the normal number of blastomeres in the sheep. J. Reprod. Fertil. 59, 357–62.CrossRefGoogle ScholarPubMed
Willadsen, S.M. (1981). The development capacity of blastomeres from 4- and 8-cell sheep embryos. J. Embryol. Exp. Morphol. 65, 165–72.Google ScholarPubMed
Willadsen, S.M. & Polge, C. (1981). Attempts to produce monozygotic quadruplets in cattle by blastomere separation. Vet. Rec. 108, 211–3.CrossRefGoogle ScholarPubMed
Willadsen, S.M., Lehn-Jensen, H., Fehilly, C.B. & Newcomb, R. (1981). The production of monozygotic twins of preselected parentage by micromanipulation of non-surgically collected cow embryos. Theriogenology 15, 23–9.CrossRefGoogle ScholarPubMed
Supplementary material: Image

Hisaki Supplementary Material

Appendix

Download Hisaki Supplementary Material(Image)
Image 1.4 MB