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Putative germline and pluripotent stem cells in adult mouse ovary and their in vitro differentiation potential into oocyte-like and somatic cells

Published online by Cambridge University Press:  03 July 2017

Yashar Esmaeilian ,
Arzu Atalay  and
Esra Erdemli
Yashar Esmaeilian*
Affiliation:
Biotechnology Institute, University of Ankara, Ankara 06500, Turkey
Arzu Atalay
Affiliation:
Biotechnology Institute, University of Ankara, Ankara, Turkey
Esra Erdemli
Affiliation:
Department of Histology and Embryology, School of Medicine, University of Ankara, Ankara, Turkey
*
All correspondence to: Yashar Esmaeilian. Biotechnology Institute, University of Ankara, Ankara 06500, Turkey. Tel: +90 312 2225816. Fax: +90 312 2225872. E-mail: [email protected]
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Summary

According to classical knowledge of reproductive biology, in the ovary of female mammals there is a limited number of oocytes and there is no possibility of renewal if the oocytes are lost due to disease or injury. However, in recent years, the results of some studies on renewal and formation of oocytes and follicles in the adult mammalian ovary have led to the questioning of this opinion. The aim of our study is to demonstrate the presence of putative germline and pluripotent stem cells in the adult mouse ovary and their differentiation potential into germ and somatic cells. In ovary tissues and cells harvested from pre-differentiation step, the expression of pluripotent and germline stem cell markers was analysed by reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence staining and western blotting. Embryoid bodies that formed in this step were analysed using immunofluorescence staining and transmission electron microscopy. Ovarian stem cells were induced to differentiate into oocyte, osteoblast, chondrocyte and neural cells. Besides morphological observation, differentiated cells were analysed by RT-PCR, histochemical and immunofluorescence staining. Expression of germline and pluripotent stem cell markers both in mRNA and at the protein level were detected in the pre-differentiated cells and ovary tissues. As a result of the differentiation process, the formation of oocyte-like cells, osteoblasts, chondrocytes and neural cells was observed and characteristics of differentiated cells were confirmed using the methods mentioned above. Our study results revealed that the adult mouse ovary contains germline and pluripotent stem cells with the capacity to differentiate into oocyte-like cells, osteoblasts, chondrocytes and neural cells.

Keywords

ChondrogenesisGermline stem cellNeurogenesisOogenesisOsteogenesisOvaryPluripotent stem cell
Type
Research Article
Information
Zygote , Volume 25 , Issue 3 , June 2017 , pp. 358 - 375
Copyright
Copyright © Cambridge University Press 2017 

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