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Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)

Published online by Cambridge University Press:  02 December 2019

Thaís T.S. Souza ,
Maria J.B. Bezerra ,
Maurício F. van Tilburg ,
Celso S. Nagano ,
Luciana D. Rola ,
José M.B. Duarte ,
Luciana M. Melo ,
Arlindo A. Moura  and
Vicente J.F. Freitas Open the ORCID record for Vicente J.F. Freitas [Opens in a new window]
Thaís T.S. Souza
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil
Maria J.B. Bezerra
Affiliation:
Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil
Maurício F. van Tilburg
Affiliation:
Health Sciences Center, State University of Ceará, Fortaleza, Brazil
Celso S. Nagano
Affiliation:
Laboratory of Mass Spectrometry, Federal University of Ceará, Fortaleza, Brazil
Luciana D. Rola
Affiliation:
Department of Animal Science, State University of São Paulo, Jaboticabal, Brazil
José M.B. Duarte
Affiliation:
Department of Animal Science, State University of São Paulo, Jaboticabal, Brazil
Luciana M. Melo
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil Molecular Genetics Research Unit, University Center Fametro, Fortaleza, Brazil
Arlindo A. Moura
Affiliation:
Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil
Vicente J.F. Freitas*
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, Brazil
*
Author for correspondence: V.J.F. Freitas. Faculty of Veterinary, State University of Ceará, Av. Dr. Silas Munguba, 1700, 60714-903, Fortaleza, Ceará, Brazil. Tel: +55 85 31019861. E-mail: [email protected]
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Summary

The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (≤3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P > 0.05) was observed between small/medium and large follicles: 49.2 ± 22.8 and 56.7 ± 27.4 μg/μl, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P > 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.

Keywords

DeerElectrophoresisMS/MSSDS-PAGE
Type
Short Communication
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 170 - 173
Copyright
© Cambridge University Press 2019

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Footnotes

*

Present address: Federal University of Paraiba, Areia, Brazil.

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