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Pronuclear formation and cleavage of mammalian eggs after microsurgical injection of freeze-dried sperm nuclei

Published online by Cambridge University Press:  26 September 2008

Kazuhiko Hoshi ,
Kaoru Yanagida ,
Haruo Katayose  and
Hiroyuki Yazawa
Kazuhiko Hoshi*
Affiliation:
Fukushima Medical College, Fukushima, Japan.
Kaoru Yanagida
Affiliation:
Fukushima Medical College, Fukushima, Japan.
Haruo Katayose
Affiliation:
Fukushima Medical College, Fukushima, Japan.
Hiroyuki Yazawa
Affiliation:
Fukushima Medical College, Fukushima, Japan.
*
K. Hoshi, Department of Obstetrics and Gynecology, Fukushima Medical College, Fukushima 960-12Japan. Tel: 0245-48-2111. Fax: 0245-48-3878.
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Summary

A study was conducted to evaluate the ability of mammalian oocytes to develop to the pronuclear stage and beyond if injected with freeze-dried sperm nuclei. The rate of development of hamster eggs to the pronuclear stage after microinjection of freeze-dried hamster sperm nuclei was 90%. The pronuclear formation rate of hamster eggs injected with freeze-dried human sperm nuclei was 85%. On the other hand, the rates for eggs injected with untreated sperm nuclei were 84% and 89% respectively. The survival rate of rabbit eggs microinjected with freeze-dried rabbit sperm nuclei was 64%, the fertilisation rate 56% and the cleavage rate 38%. The survival, fertilisation and cleavage rates of eggs injected with non-freeze-dried sperm nuclei were 78%, 45% and 34%, respectively. There was no difference between eggs injected with freeze-dried sperm nuclei and those injected with sperm nuclei that had not been freeze-dried. Of the viable rabbit eggs injected with freeze-dried rabbit sperm nuclei, 6% developed to the 6- to 8-cell stage 62 h after injection.

Keywords

CleavageFreeze-dryingMicrosurgical injectionpronulear formationSperm nucleus
Type
Article
Information
Zygote , Volume 2 , Issue 3 , August 1994 , pp. 237 - 242
Copyright
Copyright © Cambridge University Press 1994

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