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Prolonged interval time between blastocyst biopsy and vitrification compromised the outcomes in preimplantation genetic testing

Published online by Cambridge University Press:  18 February 2021

Shun Xiong Open the ORCID record for Shun Xiong [Opens in a new window] ,
Jun Xia Liu ,
Dong Yun Liu ,
Jia Hong Zhu ,
Xiang Wei Hao ,
Li Hong Wu ,
Yang Gao ,
Jing Yu Li  and
Guo Ning Huang Open the ORCID record for Guo Ning Huang [Opens in a new window]
Shun Xiong
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Jun Xia Liu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Dong Yun Liu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Jia Hong Zhu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Xiang Wei Hao
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Li Hong Wu
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Yang Gao
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
Jing Yu Li
Affiliation:
Chongqing Key Laboratory of Human Embryo Engineering, Chongqing, People’s Republic of China
Guo Ning Huang*
Affiliation:
Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China
*
Author for correspondence: Guo Ning Huang. Chongqing Health Center for Women and Children, Chongqing, People’s Republic of China. E-mail: [email protected]
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Summary

This study aimed to evaluate to what extent the different interval times between trophectoderm (TE) biopsy and vitrification influence the clinical outcomes in preimplantation genetic testing (PGT) cycles. Patients who underwent frozen embryo transfer (FET) after PGT between 2015 and 2019 were recruited. In total, 297 cycles with single day 5 euploid blastocyst transfer were included. These cycles were divided into three groups according to the interval times: <1 h group, 1–2 h group, and ≥2 h group. Blastocyst survival, clinical pregnancy, miscarriage, and ongoing pregnancy rates were compared. The results showed that, in PGT-SR cycles, survival rate in the ≥2 h group (96.72%) was significantly lower than in the <1 h group (100%, P = 0.047). The clinical pregnancy rate in the ≥2 h group was 55.93%, significantly lower than in the <1 h group (74.26%, P = 0.017). The ongoing pregnancy rates in the 1–2 h group and the ≥2 h group were 48.28% and 47.46%, respectively, significantly lower than that in the <1 h group (67.33%, P < 0.05). The miscarriage rate in the 1–2 h group was 18.42%, significantly higher than that in the <1 h group (5.33%, P = 0.027). In PGT-A cycles, the clinical pregnancy and ongoing pregnancy rates in the <1 h group were 67.44% and 53.49%, respectively, higher than that in the 1–2 h group (52.94%, 47.06%, P > 0.05) and the ≥2 h group (52.63%, 36.84%, P > 0.05). In conclusion, vitrification of blastocysts beyond 1 h after biopsy significantly influences embryo survival and clinical outcomes and is therefore not recommended.

Keywords

Blastocyst biopsyClinical pregnancy rateInterval timeOngoing pregnancy rateVitrification
Type
Research Article
Information
Zygote , Volume 29 , Issue 4 , August 2021 , pp. 276 - 281
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to the research and preparation of the manuscript.

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