Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-28T11:26:03.077Z Has data issue: false hasContentIssue false
  • English
  • Français

Pretreating porcine sperm with lipase enhances developmental competence of embryos produced by intracytoplasmic sperm injection

Published online by Cambridge University Press:  07 October 2015

Yinghui Wei ,
Junhua Fan ,
Lin Li ,
Zhiguo Liu  and
Kui Li
Yinghui Wei
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanminyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China.
Junhua Fan
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanminyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China.
Lin Li
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanminyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China.
Zhiguo Liu*
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanminyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China.
Kui Li
Affiliation:
Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanminyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China.
*
All correspondence to: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Yuanminyuan West Road No. 2, Haidian District, Beijing 100193, People's Republic of China. Tel: +86 10 62813339. Fax: +86–10–62813339. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

Intracytoplasmic sperm injection (ICSI) has been widely applied in humans, mice, and some domestic animals to cure human infertility, or produce genetically superior or genetically engineered animals. However, the production efficiency of ICSI in pigs remains quite low. In this study, we developed a new sperm pretreatment method to improve production efficiency of ICSI in pigs. Experiment 1 revealed that pretreating porcine sperm with 2.5 mg/ml lipase before ICSI operation, not only can reduce the adhesion between sperm and the injection pipette without adding polyvinylpyrrolidone (PVP) in the operating medium, but also significantly improve male pronuclei (MPN) formation rate (55.56% vs. 40.00% (0 mg/ml), 42.59% (5.0 mg/ml), 40.00% (10.0 mg/ml), P < 0.05) and enhance developmental competence of ICSI embryos (26.03% vs. 10.87% (0 mg/ml), 10.00% (5.0 mg/ml), 10.13% (10.0 mg/ml), P < 0.05). Experiment 2 showed that this method has a higher MPN formation rate (50.47% vs. 30.78%, P < 0.05) and blastocyst rate (18.81% vs. 7.41%, P < 0.05) than the PVP method, and was better than the Triton X-100 treatment method (50.47% vs. 46.23%, 18.81% vs. 12.75%). Therefore, pretreating porcine sperm with 2.5 mg/ml lipase before ICSI operation is highly recommended, instead of adding PVP in the operating medium.

Keywords

ICSIembryoLipaseTriton X-100
Type
Research Article
Information
Zygote , Volume 24 , Issue 4 , August 2016 , pp. 594 - 602
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anobom, C.D., Pinheiro, A.S., De-Andrade, R.A., Aguieiras, E.C., Andrade, G.C., Moura, M.V., Almeida, R.V. & Freire, D.M. (2014). From structure to catalysis: recent developments in the biotechnological applications of lipases. Biomed. Res. Int. 2014, 684506.Google Scholar
Catt, J.W. & Rhodes, S.L. (1995). Comparative intracytoplasmic sperm injection (ICSI) in human and domestic species. Reprod. Fertil. Dev. 7, 161–6.Google Scholar
García-Mengual, E., García-Roselló, E., Alfonso, J., Salvador, I., Cebrian-Serrano, A. & Silvestre, M. (2011). Viability of ICSI oocytes after caffeine treatment and sperm membrane removal with Triton X-100 in pigs. Theriogenology 76, 1658–66.Google Scholar
Garcia-Rosello, E., Garcia-Mengual, E., Coy, P., Alfonso, J. & Silvestre, M.A. (2009). Intracytoplasmic sperm injection in livestock species: an update. Reprod. Domest. Anim. 44, 143–51.Google Scholar
Goto, K., Kinoshita, A., Takuma, Y. & Ogawa, K. (1990). Fertilisation of bovine oocytes by the injection of immobilised, killed spermatozoa. Vet. Record 127, 517–520.Google Scholar
Hlinka, D., Herman, M., Vesela, J., Hredzak, R., Horvath, S. & Pacin, J. (1998). A modified method of intracytoplasmic sperm injection without the use of polyvinylpyrrolidone. Hum. Reprod. 13, 1922–7.Google Scholar
Hosoi, Y., Miyake, M., Utsumi, K. & Iritani, A. (1988). Development of rabbit oocytes after microinjection of spermatozoa. In Proceedings of the 11th Congress on Animal Reproduction and Artificial Insemination vol. 3, pp. 331–3.Google Scholar
Iritani, A. (1988). Current status of biotechnological studies in mammalian reproduction. Fertil. Steril. 50, 543–51.Google Scholar
Katayama, M., Koshida, M. & Miyake, M. (2002). Fate of the acrosome in ooplasm in pigs after IVF and ICSI. Hum. Reprod. 17, 2657–64.Google Scholar
Katayama, M., Sutovsky, P., Yang, B.S., Cantley, T., Rieke, A., Farwell, R., Oko, R. & Day, B. N. (2005). Increased disruption of sperm plasma membrane at sperm immobilization promotes dissociation of perinuclear theca from sperm chromatin after intracytoplasmic sperm injection in pigs. Reproduction 130, 907–16.CrossRefGoogle ScholarPubMed
Kobayashi, K., Kato, K., Saga, M., Yamane, M., Rothman, C.M. & Ogawa, S. (1992). Subzonal insemination of a single mouse spermatozoon with a personal computer controlled micromanipulation system. Mol. Reprod. Dev. 33, 81–8.Google Scholar
Lee, J. & Yang, X. (2004). Factors affecting fertilization of porcine oocytes following intracytoplasmic injection of sperm. Mol. Reprod. Dev. 68, 96–102.Google Scholar
Lessig, J., Glander, H.J., Schiller, J., Petkovic, M., Paasch, U. & Arnhold, J. (2006). Destabilization of the acrosome results in release of phospholipase A2 from human spermatozoa and subsequent formation of lysophospholipids. Andrologia 38, 69–75.Google Scholar
Li, C., Mizutani, E., Ono, T. & Wakayama, T. (2009). Production of normal mice from spermatozoa denatured with high alkali treatment before ICSI. Reproduction 137, 779–92.Google Scholar
Martin, M.J. (2000). Development of in vivo-matured porcine oocytes following intracytoplasmic sperm injection. Biol. Reprod. 63, 109–12.Google Scholar
Meng, X., Gu, X., Wu, C., Dai, J., Zhang, T., Xie, Y., Wu, Z., Liu, L., Ma, H. & Zhang, D. (2010). Effect of trehalose on the freeze-dried boar spermatozoa. Chin. J. Biotechnol. 26, 1143–9.Google Scholar
Morozumi, K. & Yanagimachi, R. (2005). Incorporation of the acrosome into the oocyte during intracytoplasmic sperm injection could be potentially hazardous to embryo development. Proc. Natl. Acad. Sci. USA 102, 14209–14.Google Scholar
Morozumi, K., Shikano, T., Miyazaki, S. & Yanagimachi, R. (2006). Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development. Proc. Natl. Acad. Sci. USA 103, 17661–6.Google Scholar
Nakai, M., Ito, J., Sato, K., Noguchi, J., Kaneko, H., Kashiwazaki, N. & Kikuchi, K. (2011). Pre-treatment of sperm reduces success of ICSI in the pig. Reproduction 142, 285–93.Google Scholar
Palermo, G., Joris, H., Devroey, P. & Van Steirteghem, A.C. (1992). Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340, 17–8.CrossRefGoogle ScholarPubMed
Parrish, J., Krogenaes, A. & Susko-Parrish, J. (1995). Effect of bovine sperm separation by either swim-up or Percoll method on success of in vitro fertilization and early embryonic development. Theriogenology 44, 859–69.Google Scholar
Perry, A.C., Wakayama, T., Kishikawa, H., Kasai, T., Okabe, M., Toyoda, Y. & Yanagimachi, R. (1999). Mammalian transgenesis by intracytoplasmic sperm injection. Science 284, 1180–3.Google Scholar
Pope, C., Johnson, C., McRae, M., Keller, G. & Dresser, B. (1998). Development of embryos produced by intracytoplasmic sperm injection of cat oocytes. Anim. Reprod. Sci. 53, 221–36.Google Scholar
Probst, S. & Rath, D. (2003). Production of piglets using intracytoplasmic sperm injection (ICSI) with flow cytometrically sorted boar semen and artificially activated oocytes. Theriogenology 59, 961–73.Google Scholar
Squires, E., Wilson, J., Kato, H. & Blaszczyk, A. (1996). A pregnancy after intracytoplasmic sperm injection into equine oocytes matured in vitro . Theriogenology 45, 306.CrossRefGoogle Scholar
Strehler, E., Baccetti, B., Sterzik, K., Capitani, S., Collodel, G., De Santo, M., Gambera, L. & Piomboni, P. (1998). Detrimental effects of polyvinylpyrrolidone on the ultrastructure of spermatozoa (Notulae seminologicae 13). Hum Reprod. 13, 120–3.Google Scholar
Tian, J., Wu, Z., Liu, L., Cai, Y., Zeng, S., Zhu, S., Liu, G., Li, Y. & Wu, C. (2006). Effects of oocyte activation and sperm preparation on the development of porcine embryos derived from in vitro-matured oocytes and intracytoplasmic sperm injection. Theriogenology 66, 439–48.Google Scholar
Wei, H. & Fukui, Y. (2000). Technical improvement in intracytoplasmic sperm injection (ICSI) in cattle. J. Reprod. Dev. 46, 403–7.Google Scholar
Wu, Z., Xing, F., Liu, G., Zeng, S., Zhu, S., Zhang, Z. & Fu, P. (2002). Studies on electrical activation of porcine oocytes matured in vitro and embryo culture systems. Scientia Agricultural Sinica (China).Google Scholar
Xiao, X. & Li, Y. (2007). The effects of pretreatment of porcine sperm with different concentration DTT or GSH on embryo development following ICSI. Prog. Vet. Med. 28, 27–32.Google Scholar
Xiao, Y., Zhang, H., Ahmad, S., Bai, L., Wang, X., Huo, L., Zhang, X., Li, W., Li, X. & Yang, L. (2013). Sperm capacitation combined with removal of the sperm acrosome and plasma membrane enhances paternal nucleus remodelling and early development of bovine androgenetic embryos. Reprod. Fertil. Dev. 25, 624–38.Google Scholar