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Parthenogenetic activation of buffalo (Bubalus bubalis) oocytes: comparison of different activation reagents and different media on their developmental competence and quantitative expression of developmentally regulated genes

Published online by Cambridge University Press:  02 October 2020

K.P. Singh ,
S.K. Mohapatra Open the ORCID record for S.K. Mohapatra [Opens in a new window] ,
R. Kaushik ,
M.K. Singh ,
P. Palta ,
S.K. Singla ,
R.S. Manik  and
M.S. Chauhan
K.P. Singh
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
S.K. Mohapatra
Affiliation:
Department of Animal Biotechnology, College of Veterinary Science and A.H. Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, Gujarat, India.
R. Kaushik
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
M.K. Singh
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
P. Palta
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
S.K. Singla
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
R.S. Manik
Affiliation:
Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India
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Summary

This study was carried out to compare the efficacy of different methods to activate buffalo A + B and C + D quality oocytes parthenogenetically and to study the in vitro developmental competence of oocytes and expression of some important genes at the different developmental stages of parthenotes. The percentage of A + B oocytes (62.16 ± 5.06%, range 53.8–71.3%) was significantly higher (P < 0.001) compared with that of C + D oocytes (37.8 ± 5.00%, range 28.6–46.1%) retrieved from slaughterhouse buffalo ovaries. Among all combinations, ethanol activation followed by culture in research vitro cleave medium gave the highest cleavage and blastocyst yields for both A + B and C + D grade oocytes. Total cell numbers, inner cell mass/trophectoderm ratio and apoptotic index of A + B group blastocysts were significantly different (P < 0.05) from their C + D counterpart. To determine the status of expression patterns of developmentally regulated genes, the expression of cumulus–oocyte complexes, fertilization, developmental competence and apoptotic-related genes were also studied in parthenogenetically produced buffalo embryos at different stages, and indicated that the differential expression patterns of the above genes had a role in early embryonic development.

Keywords

BlastocystBuffaloEmbryoOocyteParthenogenetic activation
Type
Research Article
Information
Zygote , Volume 29 , Issue 1 , February 2021 , pp. 49 - 58
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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Footnotes

*

Present address: Director, ICAR-National Dairy Research Institute, Karnal, Haryana, India.

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