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Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments

Published online by Cambridge University Press:  01 November 2007

S.C. Méo ,
W. Yamazaki ,
C.R. Ferreira ,
F. Perecin ,
N.Z. Saraiva ,
C.L.V. Leal  and
J.M. Garcia
S.C. Méo*
Affiliation:
Centro de Genética e Reprodução Animal, Instituto de Zootecnia, Rua Heitor Penteado, 56, 13460-000, Nova Odessa, SP, Brazil. Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
W. Yamazaki
Affiliation:
Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
C.R. Ferreira
Affiliation:
Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
F. Perecin
Affiliation:
Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
N.Z. Saraiva
Affiliation:
Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
C.L.V. Leal
Affiliation:
Departamento de Ciências Básicas, FZEA – USP, Avenida Duque de Caxias Norte, 225, 13635-900, Pirassununga, SP, Brazil.
J.M. Garcia
Affiliation:
Departamento de Medicina Veterinária Preventiva e Reprodução Animal, FCAV – UNESP, Via de Acesso Prof. Paulo Donato Castellane, s/n, 14884-900, Jaboticabal, SP, Brazil.
*
All correspondence to: S.C. Méo, Embrapa Pecuária Sudeste, Rodovia Washington Luiz, km 234, CEP 13560-970, São Carlos, SP, Brazil. Tel: +55 16 3361 5611. Fax: +55 16 3361 5754. e-mail: [email protected]
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Summary

In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of oocyte age on early parthenogenesis was assessed in oocytes IVM for 22, 26 and 30 h. Better results in pronuclear development were obtained in treatments ISD (81.7%) at 22 h; D (66.7%), IS (63.3%), ID (73.3%) and ISD (76.7%) at 26 h; and D (86.7%), IS (85.0%) and ID (78.3%) at 30 h. Higher cleavage occurred on ISD (80.0%) at 22 h; ID (83.3%) and ISD (91.7%) at 26 h; and I (86.7%), IS (90.0%), ID (85.0%) and ISD (95.0%) at 30 h. More blastocysts were achieved in ID (25.0%) and ISD (18.3%) at 22 h; and in ID at 26 h (45.0%) and 30 h (50.0%). We also observed that IS allowed higher haploid (77.4%) embryonic development, whilst ID was better for diploid (89.1%) development. It was concluded that association of S and D without I was not effective for blastocyst development; treatments using S were less influenced by oocyte age, but when S was associated with D there was a detrimental effect on aged oocytes; treatment ISD promoted higher activation and cleavage rates in young oocytes and ID protocol was the best for producing blastocysts.

Keywords

CattleChromatinEmbryonic developmentMicrotubulesOocyte activation
Type
Research Article
Information
Zygote , Volume 15 , Issue 4 , November 2007 , pp. 295 - 306
Copyright
Copyright © Cambridge University Press 2007

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References

Araki, K., Naito, K., Haraguchi, S., Suzuki, R., Yokoyama, M., Inoue, M., Aizawa, S., Toyoda, Y. & Sato, E. (1996). Meiotic abnormalities of c-mos knockout mouse oocytes: activation after first meiosis or entrance into third meiotic metaphase. Biol. Reprod. 55, 1315–24.CrossRefGoogle ScholarPubMed
Bos-Mikich, A., Wood, M.J., Candy, C.J. & Whittingham, D.G. (1995). Cytogenetical analysis and developmental potential of vitrified mouse oocytes. Biol. Reprod. 53, 780–5.CrossRefGoogle ScholarPubMed
Bos-Mikich, A., Whittingham, D.G. & Jones, K.T. (1997). Meiotic and mitotic Ca2+ oscillations affect cell composition in resulting blastocysts. Dev. Biol. 182, 172–9.Google Scholar
Cuthbertson, K.S.R., Whittingham, D.G. & Cobbold, P.H. (1981). Free Ca2+ increases in exponential phases during mouse oocyte activation. Nature 294, 754–7.CrossRefGoogle ScholarPubMed
De La Fuente, R. & King, W.A. (1998). Developmental consequences of karyokinesis without cytokinesis during the first mitotic cell cycle of bovine parthenotes. Biol. Reprod. 58, 952–62.Google Scholar
De Sousa, P.A., Dobrinsky, J.R., Zhu, J., Archibald, A.L., Ainslie, A., Bosma, W., Bowering, J., Bracken, J., Ferrier, P.M., Fletcher, J., Gasparrini, B., Harkness, L., Johnston, P., Ritchie, M., Ritchie, W.A., Travers, A., Albertini, D., Dinnyes, A., King, T.J. & Wilmut, I. (2002). Somatic cell nuclear transfer in the pig: control of pronuclear formation and integration with improved methods for activation and maintenance of pregnancy. Biol. Reprod. 66, 642–50.Google Scholar
Dupont, G. (1998). Link between fertilization-induced Ca2+ oscillations and relief from metaphase II arrest in mammalian eggs: a model based on calmodulin-dependent kinase II activation. Biophys. Chem. 72, 153–67.Google Scholar
Grupen, C.G., Nottle, M.B. & Nagashima, H. (2002). Calcium release at fertilization: artificially mimicking the oocyte's response to sperm. J. Reprod. Dev. 48, 313–33.Google Scholar
Heindryckx, B., Rybouchkin, A., Van Der Elst, J. & Dhont, M. (2002). Serial pronuclear transfer increases the developmental potential of in vitro-matured oocytes in mouse cloning. Biol. Reprod. 67, 1790–5.CrossRefGoogle ScholarPubMed
Kikuchi, K., Izaike, Y., Noguchi, J., Furukawa, T., Daen, F.P., Naito, K. & Toyoda, Y. (1995). Decrease of histone H1 kinase activity in relation to parthenogenetic activation of pig follicular oocytes matured and aged in vitro. J. Reprod. Fertil. 105, 325–30.CrossRefGoogle ScholarPubMed
Kim, B-K., Lee, S-C., Lee, K-S., Lee, B-K., Han, C-H., Kim, J-H. & Lee, C-S. (2002). Effect of medium milieu on sperm penetration and pronuclear formation of bovine oocytes matured in vitro. Theriogenology 57, 2093–104.CrossRefGoogle ScholarPubMed
Kishikawa, H., Wakayama, T. & Yanagimachi, R. (1999). Comparison of oocyte-activating agents for mouse cloning. Cloning 1, 153–9.Google Scholar
Koo, D-B., Kang, Y-K., Choi, Y-H., Park, J.S., Kim, H-N., Oh, K.B., Son, D-S., Park, H., Lee, K.K. & Han, Y-M. (2002). Aberrant allocations of inner cell mass and trophectoderm cells in bovine nuclear transfer blastocysts. Biol. Reprod. 67, 487–92.Google Scholar
Kubiak, J.Z. (1989). Mouse oocytes gradually develop the capacity for activation during the metaphase II arrest. Dev. Biol. 136, 537–45.CrossRefGoogle ScholarPubMed
Küpker, W., Diedrich, K. & Edwards, R.G. (1998). Principles of mammalian fertilization. Hum. Reprod. 13, 20–32.Google Scholar
Liu, L., Ju, J-C. & Yang, X. (1998). Differential inactivation of maturation-promoting factor and mitogen-activated protein kinase following parthenogenetic activation of bovine oocytes. Biol. Reprod. 59, 537–45.CrossRefGoogle ScholarPubMed
Loi, P., Ledda, S., Fulka Jr, J., Cappai, P. & Moor, R.M. (1998). Development of parthenogenetic and cloned ovine embryos: effect of activation protocols. Biol. Reprod. 58, 1177–87.CrossRefGoogle ScholarPubMed
Loren, J. & Lacham-Kaplan, O. (2006). The employment of strontium to activate mouse oocytes: effects on spermatid-injection outcome. Reproduction 131, 259–67.CrossRefGoogle ScholarPubMed
Ma, S-F., Liu, X-Y., Miao, D-Q, Han, Z-B., Zhang, X., Miao, Y-L., Yanagimachi, R. & Tan, J-H. (2005). Parthenogenetic activation of mouse oocytes by strontium chloride: a search for the best conditions. Theriogenology 64, 1142–57.Google Scholar
Malcuit, C., Maserati, M., Takahashi, Y., Page, R. & Fissore, R.A. (2006). Intracytoplasmic sperm injection in the bovine induces abnormal [Ca2+]i responses and oocyte activation. Reprod. Fertil. Dev. 18, 39–51.CrossRefGoogle ScholarPubMed
Méo, S.C., Leal, C.L.V. & Garcia, J.M. (2004). Activation and early parthenogenesis of bovine oocytes treated with ethanol and strontium. Anim. Reprod. Sci. 81, 35–46.CrossRefGoogle ScholarPubMed
Méo, S.C., Yamazaki, W., Leal, C.L.V., Oliveira, J.A. de & Garcia, J.M. (2005). Use of strontium for bovine oocyte activation. Theriogenology 63, 2089–102.Google Scholar
Nakada, K. & Mizuno, J. (1998). Intracellular calcium responses in bovine oocytes induced by spermatozoa and by reagents. Theriogenology 50, 269–82.CrossRefGoogle ScholarPubMed
Nussbaum, D.J. & Prather, R.S. (1995). Differential effects of protein synthesis inhibitors on porcine oocyte activation. Mol. Reprod. Dev. 41, 70–5.Google Scholar
Peura, T.T. & Trounson, A.O. (1998). Recycling bovine embryos for nuclear transfer. Reprod. Fertil. Dev. 10, 627–32.CrossRefGoogle ScholarPubMed
Polanski, Z., Hoffmann, S. & Tsurumi, C. (2005). Oocyte nucleus controls progression through meiotic maturation. Dev. Biol. 281, 184–95.Google Scholar
Presicce, G.A. & Yang, X. (1994). Nuclear dynamics of parthenogenesis of bovine oocytes matured in vitro for 20 and 40 hours and activated with combined ethanol and cycloheximide treatment. Mol. Reprod. Dev. 37, 61–8.Google Scholar
Saeki, R., Kato, H., Hosoi, Y., Miyake, M., Utsumi, K. & Iritani, A. (1991). Early morphological events of in vitro fertilized bovine oocytes with frozen-thawed spermatozoa. Theriogenology 35, 1051–9.CrossRefGoogle ScholarPubMed
Soloy, E., Kanka, J., Viuff, D., Smith, S.D., Callesen, H. & Greve, T. (1997). Time course of pronuclear deoxyribonucleic acid synthesis in parthenogenetically activated bovine oocytes. Biol. Reprod. 57, 27–35.CrossRefGoogle ScholarPubMed
Solter, D. (1988). Differential imprinting and expression of maternal and paternal genomes. Annu. Rev. Genet. 22, 127–46.CrossRefGoogle ScholarPubMed
Stojkovic, M., Büttner, M., Zakhartchenko, V., Brem, G. & Wolf, E. (1998). A reliable procedure for differential staining of in vitro produced bovine blastocysts: comparison of tissue culture medium 199 and Ménézo's B2 medium. Anim. Reprod. Sci. 50, 1–9.Google Scholar
Susko-Parrish, J.L., Leibfried-Rutledge, M.L., Northey, D.L., Schutzkus, V. & First, N.L. (1994). Inhibition of protein kinases after an induced calcium transient causes transition of bovine oocytes to embryonic cycles without meiotic completion. Dev. Biol. 166, 729–39.Google Scholar
Suttner, R., Zakhartchenko, V., Stojkovic, P., Müller, S., Alberio, R., Medjugorac, I., Brem, G., Wolf, E. & Stojkovic, M. (2000). Intracytoplasmic sperm injection in bovine: effects of oocyte activation, sperm pretreatment and injection technique. Theriogenology 54, 935–48.Google Scholar
Verlhac, M-H., Cúbica, J.Z., Clarke, H.J. & Maro, B. (1994). Microtubule and chromatin behavior follow MAP kinase activity by not MPF activity during meiosis in mouse oocytes. Development 120, 1017–25.CrossRefGoogle Scholar
Vitullo, A.D. & Ozil, J-P. (1992). Repetitive calcium stimuli drive meiotic resumption and pronuclear development during mouse oocyte activation. Dev. Biol. 151, 128–36.Google Scholar
Webb, M., Howlett, S.K. & Maro, B. (1986). Parthenogenesis and cytoskeletal organization in ageing mouse eggs. J. Embryol. Exp. Morphol. 95, 131–45.Google Scholar
Wells, D.N., Misica, P.M. & Tervit, H.R. (1999). Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod. 60, 996–1005.CrossRefGoogle ScholarPubMed
Xu, K.P. & Greve, T. (1988). A detailed analysis of early events during in vitro fertilization of bovine follicular oocytes. J. Reprod. Fertil. 82, 127–34.Google Scholar
Yamazaki, W., Ferreira, C.R., Méo, S.C., Leal, C.L.V., Meirelles, F.V. & Garcia, J.M. (2005). Use of strontium in the activation of bovine oocytes reconstructed by somatic cell nuclear transfer. Zygote 13, 295–302.Google Scholar
Yang, X., Presicce, G.A., Moraghan, L., Jiang, S. & Foote, R.H. (1994). Synergistic effect of ethanol and cycloheximide on activation of freshly matured bovine oocytes. Theriogenology 41, 395–403.Google Scholar