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Oxidative stress caused by a dysregulated Wnt/β-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome

Published online by Cambridge University Press:  15 October 2020

Hai Zhao Open the ORCID record for Hai Zhao [Opens in a new window] ,
Jian Zhang ,
Ning Qian ,
Shuguang Wu ,
Yanjun Wu  and
Gang Yao
Hai Zhao*
Affiliation:
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou550025, China
Jian Zhang
Affiliation:
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou550025, China
Ning Qian
Affiliation:
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou550025, China
Shuguang Wu
Affiliation:
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou550025, China
Yanjun Wu
Affiliation:
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou550025, China
Gang Yao
Affiliation:
Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou550025, China
*
Author for correspondence: Hai Zhao. Dongqing Road, Huaxi University Town, Guiyang, Guizhou550025, China. Tel: +86 0851 85652972. E-mail: [email protected]
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Summary

Chronic fatigue syndrome (CFS) is characterized by extreme fatigue and disabling symptoms. Women with CFS often have a high risk of gynaecological problems such as irregular menstruation, endometriosis and pelvic pain and sexual dysfunction. Our previous results have shown that, in pregnant mice, CFS significantly decreased the progestational hormone level in serum, as well as learning and memory, and the function of the hypothalamus–pituitary–gonadal axis. In addition, the F1 generation also suffered from congenital hypothyroidism. At present, there has been no report about placenta formation and embryonic development in pregnant mice with CFS. The aim of the present study was to investigate the influence of CFS on the morphology, oxidative stress and Wnt/β-catenin signalling pathway during placenta formation. In this study, we found that CFS decreased the number of implantation sites for blastocysts, and increased the number of absorbed, stillborn and malformed fetuses. The morphology and structure of the placenta were abnormal in pregnant mice with CFS. Further study found that the oxidative stress in serum, uterus and placenta was increased in pregnant mice with CFS, while the levels of antioxidase were decreased. CFS also inhibited the Wnt/β-catenin signalling pathway in the placenta. These results suggested that inhibition of the Wnt/β-catenin signalling pathway and enhanced oxidative stress play an important role in abnormal placentation in pregnant mice with CFS.

Keywords

Chronic fatigue syndromeOxidative stressPlacentaPregnant miceWnt/β-catenin
Type
Research Article
Information
Zygote , Volume 29 , Issue 2 , April 2021 , pp. 122 - 129
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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