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Oocyte maturation in rabbits: effects of calmodulin inhibitors

Published online by Cambridge University Press:  26 September 2008

Michael A. Henry ,
Richard G. Rawlins ,
Ewa Radwanska  and
Mary M. Fahy
Michael A. Henry*
Affiliation:
Section of Reproductive Endocrinology, Department of OB/GYN, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA
Richard G. Rawlins
Affiliation:
Section of Reproductive Endocrinology, Department of OB/GYN, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA
Ewa Radwanska
Affiliation:
Section of Reproductive Endocrinology, Department of OB/GYN, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA
Mary M. Fahy
Affiliation:
Section of Reproductive Endocrinology, Department of OB/GYN, Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL 60612, USA
*
Michael A. Henry, MD. Present address: Advanced Fertility Group, 201 Pennsylvania Parkway - Suite 205, Indianapolis, IN 46280, USA. Tel: +1 (317) 817-1300. Fax: +1 (317) 817-1306.
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Summary

Oocyte maturation in mammals follows a highly conserved pattern of release from arrest through to the extrusion of the first polar body and formation of the second metaphase spindle. Oscillations in cytoplasmic calcium concentration precede the events of maturation in many species. These calcium ions interact with and activate calcium-binding proteins, including calmodulin, within the cell. Thus, it was of interest to us to examine whether calcium acted through calmodulin in the initial stages of maturation in rabbit oocytes or whether calmodulin was required for continuation through metaphase I on to metaphase II. Using the calmodulin inhibitor W-7 we found a significant (p < 0.05) decrease in the percentage of oocytes that underwent germinal vesicle breakdown. Calmidazolium did not prevent germinal vesicle breakdown; however, it caused a significant (p < 0.05) decrease in the proportion of oocytes with fully elaborated spindles and taxol-induced cytoplasmic asters. Both inhibitors caused a significant (p < 0.05) reduction in the proportion of oocytes that extruded their first polar bodies. The kinase inhibitor 6-DMAP caused a significant reduction in the proportion of oocytes with spindles and condensed chromatin, indicating the necessity for phosphorylation events in the resumption of meiosis. In rabbit oocytes calmodulin may play a role in the release from prophase arrest, and it is necessary for spindle preservation and continuation through metaphase I to metaphase II. The varying effects of the two inhibitor stems from their different binding sites on the calmodulin molecule thus causing a differential effect on its downstream effectors.

Keywords

CalmodulinOocyte maturationRabbitSpindle
Type
Article
Information
Zygote , Volume 5 , Issue 3 , August 1997 , pp. 255 - 260
Copyright
Copyright © Cambridge University Press 1997

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