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Morphological study of apoptosis in granulosa cells and ovulation in a model of atresia in rat preovulatory follicles

Published online by Cambridge University Press:  02 October 2018

Frederico da Silva Bitecourt ,
Carina Oliveira Dumont Horta ,
Karen Santos Lima ,
Bruno Bastos Godoi ,
Fernanda Luiza Menezes Bello ,
Cíntia Maria Rodrigues ,
Luana Pereira Leite Schetino  and
Kinulpe Honorato-Sampaio Open the ORCID record for Kinulpe Honorato-Sampaio [Opens in a new window]
Frederico da Silva Bitecourt
Affiliation:
Faculdade de Medicina, Campus JK, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brasil
Carina Oliveira Dumont Horta
Affiliation:
Departamento de Fisiologia e Biofísica, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Karen Santos Lima
Affiliation:
Faculdade de Medicina, Campus JK, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brasil
Bruno Bastos Godoi
Affiliation:
Faculdade de Medicina, Campus JK, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brasil
Fernanda Luiza Menezes Bello
Affiliation:
Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Sociedade Brasileira de Fisiologia, São Paulo, Brasil
Cíntia Maria Rodrigues
Affiliation:
Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Sociedade Brasileira de Fisiologia, São Paulo, Brasil
Luana Pereira Leite Schetino
Affiliation:
Faculdade de Medicina, Campus JK, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brasil
Kinulpe Honorato-Sampaio*
Affiliation:
Faculdade de Medicina, Campus JK, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Diamantina, Brasil Programa Multicêntrico de Pós-Graduação em Ciências Fisiológicas, Sociedade Brasileira de Fisiologia, São Paulo, Brasil
*
Author for correspondence: Kinulpe Honorato-Sampaio, Faculdade de Medicina, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Campus JK, Rodovia MGT 367, Km 583, no. 5000, Alto da Jacuba, Diamantina/MG, Brazil. 39100–000. Tel: +55 31 3532 1201. E-mail: [email protected]
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Summary

Previous studies have established a model of atresia in preovulatory follicles after stimulation of immature rats with equine chorionic gonadotropin (eCG). This gonadotropin recruits a follicular pool and the deprivation of preovulatory luteinizing hormone (LH) surge induces the atresia in preovulatory follicles. The present study investigated the occurrence of ovulation and provided some morphological features of granulosa cell (GC) apoptosis of atretic follicles at 0, 48, 72 and 120 h after eCG stimulation. Histological sections of ovaries from untreated animals (0 h) showed primordial, primary, secondary and early antral follicles. After 48 h ovaries showed large antral follicles. Preovulatory follicles were observed at 72 h, and two out of five rats displayed cumulus–oocyte complexes (COCs) in the oviducts. All animals exhibited corpora lutea after 120 h. We observed increased estradiol (E2) levels 48 h after eCG treatment that might trigger an endogenous preovulatory gonadotropin surge. Higher progesterone (P4) level, which is the hallmark of a functional corpus luteum, was observed at 120 h. Atresia in secondary and antral follicles was observed by pyknotic granulosa cell nuclei in histology and positive immunolabelling for cleaved caspase 3. We also observed macrophages in secondary and antral follicles in atresia. Transmission electron microscopy revealed GCs with compacted chromatin against the nuclear envelope, nuclear fragmentation, cell shrinkage and fragmentation. No preovulatory follicles showed apoptosis of GCs. In conclusion, our results suggested the occurrence of an endogenous gonadotropin surge, promoting ovulation and preventing atresia of preovulatory follicles.

Keywords

AtresiaGonadotropinGranulosa cell apoptosisOvulationPreovulatory follicle
Type
Short Communication
Information
Zygote , Volume 26 , Issue 4 , August 2018 , pp. 336 - 341
Copyright
© Cambridge University Press 2018 

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